Multiplex Assay Kit for Albumin (ALB) ,etc. by CBA (Cytometric Bead Array) 
Serum albumin
Overview
Properties
- Product No.CMB028Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryTumor immunityInfection immunityHematologyHepatologyNutrition metabolism
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Albumin (ALB) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Albumin (ALB) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 89-97 | 94 |
| EDTA plasma(n=5) | 94-101 | 97 |
| heparin plasma(n=5) | 91-104 | 96 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Albumin (ALB) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Albumin (ALB) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Albumin (ALB) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-96% | 93-101% | 80-93% | 96-104% |
| EDTA plasma(n=5) | 87-95% | 97-105% | 78-89% | 84-101% |
| heparin plasma(n=5) | 80-105% | 82-95% | 91-98% | 95-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:ALB) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Albumin (ALB) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Albumin (ALB) ,etc. by CBA (Cytometric Bead Array) and unlabeled Albumin (ALB) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Albumin (ALB) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Albumin (ALB) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Albumin (ALB) ,etc. by CBA (Cytometric Bead Array) in the sample.
Giveaways
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Citations
- Simvastatin Reduces Endotoxin-Induced Acute Lung Injury by Decreasing Neutrophil Recruitment and Radical FormationPubMed: 22701728
- Albumin administration protects against bilirubin-induced auditory brainstem dysfunction in Gunn rat pupsOnlinelibrary: liv.12219
- Adjunctive corticosteroid therapy improves lung immunopathology and survival during severe secondary pneumococcal pneumonia in micePubmed: 24273183
- Increased Circulating Levels of Vitamin D Binding Protein in MS PatientsPubmed:25590278
- The wnt/β-catenin signaling pathway participates in rhein ameliorating kidney injury in DN micePubMed: 26346164
- Collecting Protein Biomarkers in Breath Using Electret Filters: A Preliminary Method on New Technical Model and Human StudyPubmed:26934615
- 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineeringart00007
- Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy.pubmed:29246788
- Resolvin D1 Promotes SIRT1 Expression to Counteract the Activation of STAT3 and NF-κB in Mice with Septic-Associated Lung InjuryPubmed:30014231
- Maternal low-quality protein diet exerts sex-specific effects on plasma amino acid profile and alters hepatic expression of methyltransferases in adult rat offspringPubmed:29582727
- Electret filter collects more exhaled albumin than glass condenser: A method comparison based on human studyPubmed:29384875
- Therapeutic efficiency of human amniotic epithelial stem cell-derived functional hepatocyte-like cells in mice with acute hepatic failurePubmed: 30463600
- Decellularized Human Liver Scaffold‐Based Three‐dimensional Culture System Facilitate HBV InfectionPubmed: 30963688
- Treatment with 3, 4-dihydroxyphenylethyl alcohol glycoside ameliorates sepsis-induced ALI in mice by reducing inflammation and regulating M1 polarizationPubmed: 31146107
- Anti-proteinuria effect of antibody against ANGPTL3 coil-coiled domain on adriamycin-induced nephropathy in micePubmed: 31256934
- A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activationPubmed: 31447572
- Diiodothyronines regulate metabolic homeostasis in primary human hepatocytes by modulating mTORC1 and mTORC2 activityPubmed: 31580898
- Preliminary study on the effects of enrofloxacin, flunixin meglumine and pegbovigrastim on Mycoplasma bovis pneumoniaPubmed: 31655595
- Ghrelin alleviates traumatic brain injury-induced acute lung injury through pyroptosis/NF-κB pathwayPubmed: 31918060
- Sodium butyrate ameliorates deoxycorticosterone acetate/salt-induced hypertension and renal damage by inhibiting the MR/SGK1 pathwayPubmed: 32908237
- Sleeve Gastrectomy Attenuates Diabetic Nephropathy by Upregulating Nephrin Expressions in Diabetic Obese RatsPubmed: 32399849
- Protective Role of Training and Selenium Consumption Against Renal Toxicity Induced by Cadmium in Rats
- Gut Dysbiosis Contribute to High Fructose Induced Salt Sensitive Hypertension In Sprague-Dawley Rats
- A light-induced self-powered competitive immunosensor for the detection of platelet derived growth factor-BB via an elaborately assembled bioconjugate
- cGAS promotes sepsis in radiotherapy of cancer by up-regulating caspase-11 signaling33721834
- A simple and sensitive electrochemical impedimetric immunosensor for the detection of apolipoprotein E4 based on CeZnO nanoparticle
- Syringaresinol Resisted Sepsis-Induced Acute Lung Injury by Suppressing Pyroptosis Via the Oestrogen Receptor-β Signalling Pathway34807349
- GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity34853313
- Emitter–Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions34914377
- Moderation of gut microbiota and bile acid metabolism by chlorogenic acid improves high-fructose-induced salt-sensitive hypertension in micePubmed:35708145
- Syringaresinol attenuates kidney injury by inhibition of pyroptosis via activating the Nrf2 antioxidant pathway in diabetic nephropathy