Multiplex Assay Kit for Aldosterone (ALD) ,etc. by CBA (Cytometric Bead Array) Pan-species (General) Protein, Antibody, Assaykit, Service

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 669.00 US$ 955.00
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  • Multiplex Assay Kit for Aldosterone (ALD) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Aldosterone (ALD) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of Aldosterone (ALD) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Aldosterone (ALD) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-96 88
EDTA plasma(n=5) 78-93 81
heparin plasma(n=5) 90-101 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aldosterone (ALD) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aldosterone (ALD) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Aldosterone (ALD) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-98% 81-103% 93-102% 86-96%
EDTA plasma(n=5) 78-102% 89-96% 95-102% 98-105%
heparin plasma(n=5) 81-94% 93-101% 81-104% 81-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:ALD) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Aldosterone (ALD) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Aldosterone (ALD) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Aldosterone (ALD) ,etc. by CBA (Cytometric Bead Array) and unlabeled Aldosterone (ALD) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Aldosterone (ALD) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Aldosterone (ALD) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Aldosterone (ALD) ,etc. by CBA (Cytometric Bead Array) in the sample.

Giveaways

Increment services

Citations

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  • Relevance of a Hypersaline Sodium-Rich Naturally Sparkling Mineral Water to the Protection against Metabolic Syndrome Induction in Fructose-Fed Sprague-Dawley Rats: A Biochemical, Metabolic, and Redox ApproachHindawi: 384583
  • Relevance of a Hypersaline Sodium-Rich Naturally Sparkling Mineral Water to the Protection against Metabolic Syndrome Induction in Fructose-Fed Sprague-Dawley Rats: A Biochemical, Metabolic, and Redox Approach.Pubmed:24672546
  • Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorderPubmed:26934364
  • H,K-ATPase type 2 contributes to salt-sensitive hypertension induced by K(+) restriction.pubmed:27562425
  • Magnitude of Resistant Hypertension and Impact of Aldosterone to Renin Ratio In Resistant Hypertensionpublication:311777221
  • Effects of renal denervation on monocrotaline induced pulmonary remodelingpubmed:28187460
  • Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathypubmed:29240788
  • Regeneration of Functional Adrenal Tissue Following Bilateral Adrenalectomy.pubmed:29059290
  • Acute adrenal cortex injury during cardiopulmonary bypass in a canine modelPubmed:29753511
  • ANP-stimulated sodium secretion in the collecting duct prevents sodium retention in renal adaptation to acid loadPubmed: 31188029
  • MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression ModelPubmed: 31540304
  • Hyperinsulinemia rather than insulin resistance itself induces blood pressure elevation in high fat diet-fed ratsPubmed: 32349626
  • Preventive preclinical efficacy of intravenously administered sphingosine-1-phosphate (S1P) in strengthening hypoxia adaptive responses to acute and sub-chronic …
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