Multiplex Assay Kit for Alkaline Phosphatase, Tissue-nonspecific (ALPL) ,etc. by CBA (Cytometric Bead Array) Homo sapiens (Human) Protein, Antibody, Assaykit, Service

BALP; BSAP; HOPS; AP-TNAP; TNSALP; Bone Alkaline Phosphatase; Alkaline Phosphatase,Tissue-Nonspecific Isozyme; Alkaline Phosphatase, Liver/Bone/Kidney

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 559.00 US$ 798.00
  • Quantity
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  • Multiplex Assay Kit for Alkaline Phosphatase, Tissue-nonspecific (ALPL) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Alkaline Phosphatase, Tissue-nonspecific (ALPL) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Alkaline Phosphatase, Tissue-nonspecific (ALPL) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Alkaline Phosphatase, Tissue-nonspecific (ALPL) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-102 89
EDTA plasma(n=5) 78-102 90
heparin plasma(n=5) 86-94 91

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alkaline Phosphatase, Tissue-nonspecific (ALPL) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alkaline Phosphatase, Tissue-nonspecific (ALPL) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alkaline Phosphatase, Tissue-nonspecific (ALPL) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-103% 98-105% 92-101% 93-105%
EDTA plasma(n=5) 87-95% 82-91% 89-98% 83-101%
heparin plasma(n=5) 80-89% 88-95% 83-90% 78-94%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Alkaline Phosphatase, Tissue-nonspecific (ALPL) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Alkaline Phosphatase, Tissue-nonspecific (ALPL) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Alkaline Phosphatase, Tissue-nonspecific (ALPL) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Alkaline Phosphatase, Tissue-nonspecific (ALPL) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Alkaline Phosphatase, Tissue-nonspecific (ALPL) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • Osteopenic bone cell response to strontium-substituted hydroxyapatiteSpringerLink: n8233337w171v451
  • Dextromethorphan inhibits osteoclast differentiation by suppressing RANKL-induced nuclear factor-κB activationPubMed: 23400250
  • Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in miceWiley: Source
  • The antidepressant bupropion exerts alleviating properties in an ovariectomized osteoporotic rat modelPubmed:25544359
  • Enhanced differentiation of osteoblastic cells on novel chitosan/β-1, 3-glucan/bioceramic scaffolds for bone tissue regenerationPubmed:25586067
  • Effect of Mirtazapine on Rat Bone Tissue after OrchidectomyPubMed: 25871861
  • New method for the fabrication of highly osteoconductive β-1,3-glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological characterization.Pubmed:27239050
  • MiR-142-5p promotes bone repair by maintaining osteoblast activityPubmed:27085967
  • New method for the fabrication of highly osteoconductive β‐1, 3‐glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological …doi:10.1002
  • MiR‑142‑5p promotes bone repair by maintaining osteoblast activitypubmed:27085967
  • Hydrolysis of Extracellular Pyrophosphate increases in post-hemodialysis plasmaPubmed:30038263
  • Suppression Effect of Astaxanthin on Osteoclast Formation In Vitro and Bone Loss In VivoPubmed:29562730
  • Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive RatsPubmed:29898457
  • Combination therapy with BMP‑2 and psoralen enhances fracture healing in ovariectomized mice10.3892:etm.2018.6353
  • Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9
  • The effect of low temperature atmospheric nitrogen plasma on MC3T3-E1 preosteoblast proliferation and differentiation in vitro
  • Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect34104195

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