Multiplex Assay Kit for Alpha-2-Heremans Schmid Glycoprotein (AHSG) ,etc. by CBA (Cytometric Bead Array) 
aHSG; HSGa; A2HS; AHS; FETUA; HSG-A2; Fetuin-A; Alpha 2-HS Glycoprotein; Fetuin A; Alpha-2-Z-Globulin; Ba-alpha-2-Glycoprotein
Overview
Properties
- Product No.CMA178Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryInfection immunity
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Alpha-2-Heremans Schmid Glycoprotein (AHSG) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Alpha-2-Heremans Schmid Glycoprotein (AHSG) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-101 | 96 |
| EDTA plasma(n=5) | 92-99 | 95 |
| heparin plasma(n=5) | 84-94 | 88 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-2-Heremans Schmid Glycoprotein (AHSG) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-2-Heremans Schmid Glycoprotein (AHSG) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-2-Heremans Schmid Glycoprotein (AHSG) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-102% | 79-91% | 95-102% | 87-103% |
| EDTA plasma(n=5) | 93-104% | 96-104% | 98-105% | 88-102% |
| heparin plasma(n=5) | 79-90% | 87-94% | 78-105% | 79-96% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Alpha-2-Heremans Schmid Glycoprotein (AHSG) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Alpha-2-Heremans Schmid Glycoprotein (AHSG) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Alpha-2-Heremans Schmid Glycoprotein (AHSG) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Alpha-2-Heremans Schmid Glycoprotein (AHSG) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.
Giveaways
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Citations
- Proteomic analysis reveals oxidative stress response as the main adaptative physiological mechanism in cows under different production systemsScienceDirect: S1874391912002084
- Curcumin prevents liver fat accumulation and serum fetuin-A increase in rats fed a high-fat dietPubMed: 23430567
- Serum Fetuin-A Levels Related with Microalbuminuria in Diet-Induced Obese RatsPubMed: PMC3591142
- Estrogen increases the transcription of human α2-Heremans-Schmid-glycoprotein by an interplay of estrogen receptor α and activator protein-1.Pubmed: 24504099
- A novel 11β-hydroxysteroid dehydrogenase type1 inhibitor CNX-010-49 improves hyperglycemia, lipid profile and reduces body weight in diet induced obese C57B6/J mice with a potential to provide cardio protective benefitsPubmed:25098735
- Relationship Between Serum Fetuin-A Levels and Carotid Intima-media Thickness in Turkish Patients with Mild to Moderate Psoriasis. A Case-control StudyPubMed: 26476900
- Fetuin A promotes lipotoxicity in β cells through the TLR4 signaling pathway and the role of pioglitazone in anti-lipotoxicity.PubMed: 25986658
- Evaluation of Arterial Stiffness in Patients with BehPubMed: 26719639
- Visceral and subcutaneous adipose tissue express and secrete functional alpha2hsglycoprotein (fetuin a) especially in obesitypubmed:27738888
- Targeted Microbiome Intervention by Microencapsulated Delayed-Release Niacin Beneficially Affects Insulin Sensitivity in Humanspubmed:29212824
- Acute Anti-Inflammatory Markers ITIH4 and AHSG in Mice Brain of a Novel Alzheimer's Disease ModelPubmed: 30958362
- A New Serum Biomarker Set to Detect Mild Cognitive Impairment and Alzheimer's Disease by Peptidome TechnologyPubmed: 31771070
- Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats33833309
- Predıctıve value of pregnancy of follıcular fluıd fetuın‐A and‐B levels ın infertıle women after intra‐cytoplasmic sperm injection34708901