Multiplex Assay Kit for Amyloid Beta Peptide 1-40 (Ab1-40) ,etc. by CBA (Cytometric Bead Array) 
Overview
Properties
- Product No.CMA864Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryInfection immunityNeuro science
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Amyloid Beta Peptide 1-40 (Ab1-40) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Amyloid Beta Peptide 1-40 (Ab1-40) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 89-97 | 92 |
| EDTA plasma(n=5) | 78-99 | 84 |
| heparin plasma(n=5) | 79-101 | 82 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amyloid Beta Peptide 1-40 (Ab1-40) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amyloid Beta Peptide 1-40 (Ab1-40) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Amyloid Beta Peptide 1-40 (Ab1-40) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 99-105% | 98-105% | 85-99% | 80-94% |
| EDTA plasma(n=5) | 82-97% | 87-101% | 81-89% | 78-97% |
| heparin plasma(n=5) | 88-99% | 97-105% | 90-99% | 79-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:Ab1-40) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Amyloid Beta Peptide 1-40 (Ab1-40) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Amyloid Beta Peptide 1-40 (Ab1-40) ,etc. by CBA (Cytometric Bead Array) and unlabeled Amyloid Beta Peptide 1-40 (Ab1-40) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Amyloid Beta Peptide 1-40 (Ab1-40) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Amyloid Beta Peptide 1-40 (Ab1-40) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Amyloid Beta Peptide 1-40 (Ab1-40) ,etc. by CBA (Cytometric Bead Array) in the sample.
Giveaways
Increment services
Citations
- Metabolites of Cerebellar Neurons and Hippocampal Neurons Play Opposite Roles in Pathogenesis of Alzheimer's DiseasePubMed: PMC2677455
- Antagonist of peroxisome proliferator-activated receptor γ induces cerebellar amyloid-β levels and motor dysfunction in APP/PS1 transgenic miceScienceDirect: S0006291X09008468
- Effect of 900 MHz Radio Frequency Radiation on Beta Amyloid Protein, Protein Carbonyl, and Malondialdehyde in the BrainInforma: 15368378.2011.624654
- Do 100- and 500-μT ELF magnetic fields alter beta-amyloid protein, protein carbonyl and malondialdehyde in rat brains?PubMed: 23324065
- Intranasal administration of insulin lowers amyloid-levels in rat model of diabetes.PubMed: 22279939
- The γ-secretase inhibitor 2-[(1R)-1-[(4-chlorophenyl)sulfonyl](2,5-difluorophenyl) amino]ethyl-5-fluorobenzenebutanoic acid (BMS-299897) alleviates Aβ1-42 seeding and short-term memory deficits in the Aβ25-35 mouse model of Alzheimer's disease.ScienceDirect: S0014299912009028
- Blockade of Tau Hyperphosphorylation and Aβ1–42 Generation by the Aminotetrahydrofuran Derivative ANAVEX2-73, a Mixed Muscarinic and σ1 Receptor Agonist, in a Nontransgenic Mouse Model of Alzheimer’s DiseasePubmed: 23493042
- 慢病毒介导的ＣＢＳ基因过表达对原代皮质神经元Globesci:Source
- Effects of Aluminium on β-Amyloid (1–42) and Secretases (APP-Cleaving Enzymes) in Rat BrainPubmed:24792732
- Geniposide attenuates insulin-deficiency-induced acceleration of β-amyloidosis in an APP/PS1 transgenic model of Alzheimer's diseasePubMed: 25882165
- The Potential Effect of Epigallocatechin-3-Gallate alone or in Combination with Vitamin E and Selenium on Alzheimer's Disease Induced by Aluminum in Ratsopen-access:
- Tong Luo Jiu Nao, a Chinese Medicine Formula, Reduces Inflammatory Stress in a Mouse Model of Alzheimer's DiseasePubmed:26993489
- Norovirus P particle-based active Aβ immunotherapy elicits sufficient immunogenicity and improves cognitive capacity in a mouse model of Alzheimer’s diseasearticles:srep41041.ris
- Mechanisms of DHA-enriched phospholipids in improving cognitive deficits in aged SAMP8 mice with high-fat dietPubmed:29986309
- Effects of Astaxanthin and Docosahexaenoic-Acid-Acylated Astaxanthin on Alzheimer's Disease in APP/PS1 Double-Transgenic MicePubmed:29695154
- Amelioration by nitric oxide (NO) mimetics on neurobehavioral and biochemical changes in experimental model of Alzheimer's disease in ratsPubmed:29522778
- Deletion of plasma Phospholipid Transfer Protein (PLTP) increases microglial phagocytosis and reduces cerebral amyloid-β deposition in the J20 mouse …Pubmed:29731975
- Ellagic acid ameliorates learning and memory impairment in APP/PS1 transgenic mice via inhibition of β‑amyloid production and tau hyperphosphorylationDoi: 10.3892/etm.2018.6860
- 非编码RNA miR-137 调控tau 蛋白磷酸化和L 型钙离子通道CACNA1C 参与阿尔茨海默病的发生
- Effect of Aluminum-Maltolate on the Content of Aβ Protein and the Expression of ApoER2, VLDLRs, and LRP1 in PC12-ApoE4 CellsPubmed: 30649678
- Obstructive sleep apnea may increase the risk of Alzheimer's diseasePubmed: 31487291
- Prevention of Early Alzheimer's Disease by Erinacine A-Enriched Hericium erinaceus Mycelia Pilot Double-Blind Placebo-Controlled StudyPubmed: 32581767
- PYRIMIDINE-4H-1OH DERIVATIVES RESTORE MITOCHONDRIAL FUNCTION IN EXPERIMENTAL CHRONIC TRAUMATIC ENCEPHALOPATHY
- Mahanimbine-induced neuroprotection via cholinergic system and attenuated amyloidogenesis as well as neuroinflammation in lipopolysaccharides-induced …
- Lovastatin attenuates sevoflurane-induced cognitive disorder in aged rats via reducing A¦Â accumulation34048842
- Immunization with A¦Â3-10-KLH vaccine improves cognitive function and ameliorates mitochondrial dysfunction and reduces Alzheimer's disease-like pathology in Tg ¡34044034