Multiplex Assay Kit for Angiopoietin Like Protein 8 (ANGPTL8) ,etc. by CBA (Cytometric Bead Array) Homo sapiens (Human) Protein, Antibody, Assaykit, Service

RIFL; TD26; C19orf80; Lipasin; Betatrophin; Hepatocellular Carcinoma-Associated Protein TD26

Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • Multiplex Assay Kit for Angiopoietin Like Protein 8 (ANGPTL8) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Angiopoietin Like Protein 8 (ANGPTL8) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Angiopoietin Like Protein 8 (ANGPTL8) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Angiopoietin Like Protein 8 (ANGPTL8) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-98 90
EDTA plasma(n=5) 84-93 89
heparin plasma(n=5) 92-101 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiopoietin Like Protein 8 (ANGPTL8) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiopoietin Like Protein 8 (ANGPTL8) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiopoietin Like Protein 8 (ANGPTL8) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-104% 83-102% 99-105% 78-89%
EDTA plasma(n=5) 82-102% 80-93% 85-95% 99-105%
heparin plasma(n=5) 88-95% 79-88% 92-105% 82-90%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Angiopoietin Like Protein 8 (ANGPTL8) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Angiopoietin Like Protein 8 (ANGPTL8) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Angiopoietin Like Protein 8 (ANGPTL8) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Angiopoietin Like Protein 8 (ANGPTL8) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Angiopoietin Like Protein 8 (ANGPTL8) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • Increased Maternal and Cord Blood Betatrophin in Gestational DiabetesPubMed: 26115519
  • Serum betatrophin level increased in subjects with metabolic syndrome: a case-control studywp-content:10563
  • Serum betatrophin level increased in subjects with nonalcoholic fatty liver diseasefiles:ijcem0020951.pdf
  • Resolving Discrepant Findings on ANGPTL8 in β-Cell Proliferation: A Collaborative Approach to Resolving the Betatrophin Controversypubmed:27410263
  • Relationship between serum betatrophin levels and the first-phase of glucose-stimulated insulin secretionpubmed:27554132
  • Aumento del nivel de betatrofina en suero en sujetos con síndrome metabólico: estudio de casos y controles:
  • Levels of betatrophin decrease during pregnancy despite increased insulin resistance, beta-cellfunction and triglyceride levels.pubmed:27555469
  • Decreased circulating levels of betatrophin in Chinese women with polycystic ovary syndrome:
  • Serum concentrations of betatrophin and its association with indirect indices of insulin resistance and beta cell function in women with polycystic ovary …pubmed:28702052
  • Angiopoietin-like protein 8: an attractive biomarker for the evaluation of subjects with insulin resistance and related disorderspubmed:28965028
  • ANGPTL8 (Betatrophin) Role in Diabetes and Metabolic Diseasespubmed:28722798
  • The Correlation Between Circulating Betatrophin and Insulin Resistance in General Population: A Meta-Analysispubmed:28931172
  • ANGPTL8: an important regulator in metabolic disordersPubmed:29719529
  • ChREBP Reciprocally Regulates Liver and Plasma Triacylglycerol Levels in Different MannersPubmed: 30405056
  • Angptl8 mediates food-driven resetting of hepatic circadian clock in micePubmed: 31388006
  • The Impact of Psoriasis and Metabolic Syndrome on the Systemic Inflammation and Oxidative Damage to Nucleic AcidsPubmed: 32537470
  • Genetic and Metabolic Determinants of Plasma Levels of ANGPTL833619548
  • Association of betatrophin amounts with 25-(OH) D levels in patients with gestational diabetes mellitus33879746
  • Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet?¡­34035808

Recommend products