Multiplex Assay Kit for Annexin A2 (ANXA2) ,etc. by CBA (Cytometric Bead Array) Mus musculus (Mouse) Protein, Antibody, Assaykit, Service

ANX-A2; ANX2L4; CAL1H; LIP2; LPC2; LPC2D; P36; PAP-IV; Protein I; Chromobindin-8; Calpactin-1 heavy chain; Lipocortin II; Placental anticoagulant protein IV

Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • Multiplex Assay Kit for Annexin A2 (ANXA2) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Annexin A2 (ANXA2) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Annexin A2 (ANXA2) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Annexin A2 (ANXA2) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-104 96
EDTA plasma(n=5) 97-105 102
heparin plasma(n=5) 91-102 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A2 (ANXA2) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A2 (ANXA2) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Annexin A2 (ANXA2) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-98% 95-104% 93-103% 93-102%
EDTA plasma(n=5) 92-105% 89-101% 95-102% 88-98%
heparin plasma(n=5) 84-96% 87-95% 86-103% 79-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Annexin A2 (ANXA2) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Annexin A2 (ANXA2) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Annexin A2 (ANXA2) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Annexin A2 (ANXA2) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Annexin A2 (ANXA2) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • Subcellular proteome analysis unraveled annexin A2 related to immune liver fibrosisPubMed: 20225235
  • Expression characteristics and diagnostic value of annexin A2 in hepatocellular carcinomaPubMed: PMC3491596
  • Annexin A2 is not a good biomarker for hepatocellular carcinoma in cirrhosisPubMed: PMC3742823
  • Annexin A2 Versus Afp as An Efficient Diagnostic Serum Marker for Hepatocellular CarcinomaGhrnet: Source
  • Serum annexin A2 levels in acute brucellosis and brucellar spondylodiscitisPubmed:24853056
  • Circulating annexin A2 as a biomarker in gastric cancer patients: Correlation with clinical variablesPubmed:25661364
  • Serum Levels of Annexin A2 as a Candidate Biomarker for Hepatic Fibrosis in Patients With Chronic Hepatitis BPubMed: 26587036
  • Up-regulation of annexin A2 expression predicates advanced clinicopathological features and poor prognosis in hepatocellular carcinomaPubMed: 26109000
  • CirculatingPubMed: 25661364
  • Evaluation of annexin A2 and as potential biomarkers for hepatocellular carcinomaPubMed: 26189841
  • Stairs instead of elevators at the workplace decreases PCSK9 levels in a healthy populationPubMed: 26081791
  • Serum Annexin A2 Level Is Associated With Diagnosis and Prognosis in Patients With OralSquamous Cell Carcinoma.pubmed:27889534
  • Assessment of annexin A5 and annexin A2 levels as biomarkers for pre-eclampsia: A pilot study.pubmed:28501283
  • Epigallocatechin gallate induces an up-regulation of LDL receptor accompanied by a reduction of PCSK9 via the annexin A2-independent pathway in HepG2 cells.pubmed:28181408
  • Expression of annexin A2 in adenomyosis and dysmenorrheaPubmed: 31183557
  • Extracellular Vesicles-Based Biomarkers Represent a Promising Liquid Biopsy in Endometrial CancerPubmed: 31842290
  • PLASMA BIOMARKER FOR OVARIAN CANCER
  • Diagnostic Value of Plasma Annexin A2 in Early-Stage High-Grade Serous Ovarian Cancer. Diagnostics 2021, 11, 6933406648
  • Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C
  • Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins
  • Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression34376212

Recommend products