Multiplex Assay Kit for Apolipoprotein B100 (APOB100) ,etc. by CBA (Cytometric Bead Array) 
Apo-B100
- UOM
- FOB US$ 474.00 US$ 492.00 US$ 520.00 US$ 556.00 US$ 593.00 US$ 648.00 US$ 730.00 US$ 912.00
- Quantity
Overview
Properties
- Product No.CMA603Rb
- Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathwayEndocrinologyCardiovascular biologyHepatologyNutrition metabolism
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Apolipoprotein B100 (APOB100) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Apolipoprotein B100 (APOB100) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 91-101 | 96 |
| EDTA plasma(n=5) | 93-101 | 98 |
| heparin plasma(n=5) | 97-105 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein B100 (APOB100) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein B100 (APOB100) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Apolipoprotein B100 (APOB100) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-97% | 93-101% | 87-104% | 84-97% |
| EDTA plasma(n=5) | 80-88% | 81-103% | 84-95% | 88-96% |
| heparin plasma(n=5) | 81-99% | 88-102% | 84-105% | 84-93% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Apolipoprotein B100 (APOB100) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Apolipoprotein B100 (APOB100) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Apolipoprotein B100 (APOB100) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Apolipoprotein B100 (APOB100) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.
Giveaways
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Citations
- Enhancement of Naringenin Bioavailability by Complexation with Hydroxypropoyl-β-CyclodextrinPlosone: 0018033
- Chemical sympathectomy induces arterial accumulation of native and oxidized LDL in hypercholesterolemic ratsScienceDirect: S1566070211004152
- iTRAQ-based proteomic profiling of human serum reveals down-regulation of platelet basic protein and apolipoprotein B100 in patients with hematotoxicity induced by chronic occupational benzene exposureScienceDirect: S0300483X11004628
- Up-reCavia (Guinea pig )lation of Hnf1α gene expression in the liver of rats with experimentally induced chronic renal failure – A possible link between circulating PCSK9 and triacylglycerol concentrations Pubmed:26978583
- Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patientsPubmed:26680509
- Alleviating VLDL overproduction is an important mechanism for Laminaria japonica polysaccharide to inhibit atherosclerosis in LDLr-/- mice with diet-induced insulin resistance.pubmed:27928899
- Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients.pubmed:26680509
- Choline and betaine ameliorate liver lipid accumulation induced by vitamin B6 deficiency in rats.pubmed:27696964
- Accumulation of Liver Lipids Induced by Vitamin B6 Deficiency Was Effectively Ameliorated by Choline and, to a Lesser Extent, BetainePubmed: 30814419
- Zygophyllum album saponins prevent atherogenic effect induced by deltamethrin via attenuating arterial accumulation of native and oxidized LDL in ratsPubmed: 32105945
- Permethrin induced arterial retention of native and oxidized LDL in rats by promoting inflammation, oxidative stress and affecting LDL receptors, and collagen …Pubmed: 32911180
- GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity34853313
- Postprandial dyslipidemia after a standardized high-fat meal in BMI-matched healthy individuals, and in subjects with prediabetes or Type 2 diabetes34656950
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