Multiplex Assay Kit for Aspartate Aminotransferase (AST) ,etc. by CBA (Cytometric Bead Array) Sus scrofa; Porcine (Pig) Protein, Antibody, Assaykit, Service

cCAT; AAT; ASAT; SGOT; GOT1; Cysteine transaminase, cytoplasmic; Transaminase A; Aspartate Transaminase 1,Cytoplasmic; Glutamic-Oxaloacetic Transaminase 1,Soluble

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 671.00 US$ 958.00
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  • Multiplex Assay Kit for Aspartate Aminotransferase (AST) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Aspartate Aminotransferase (AST) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Aspartate Aminotransferase (AST) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Aspartate Aminotransferase (AST) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 94-105 101
EDTA plasma(n=5) 94-101 97
heparin plasma(n=5) 84-99 91

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aspartate Aminotransferase (AST) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aspartate Aminotransferase (AST) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Aspartate Aminotransferase (AST) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-92% 82-105% 91-105% 92-101%
EDTA plasma(n=5) 84-92% 95-104% 81-101% 80-102%
heparin plasma(n=5) 96-103% 91-99% 90-97% 84-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Aspartate Aminotransferase (AST) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Aspartate Aminotransferase (AST) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Aspartate Aminotransferase (AST) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Aspartate Aminotransferase (AST) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Aspartate Aminotransferase (AST) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and -independent mechanismsPubMed: PMC3612362
  • Pathogenesis of emerging severe fever with thrombocytopenia syndrome virus in C57/BL6 mouse modelPubMed: PMC3382536
  • Protective effect of Panax ginseng against N-acetyl-p-aminophenol-induced hepatotoxicity in ratsAcademicjournals: Source
  • Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and-independent mechanismsPubmed: 23325074
  • Protective effect of Et-1 receptor antagonist bosentan on paracetamol induced acute liver toxicity in ratsScienceDirect: S0014299914000235
  • The P2X1 Receptor Is Required for Neutrophil Extravasation during Lipopolysaccharide-Induced Lethal Endotoxemia in MicePubmed:25480563
  • Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol PoisoningPubMed: 26697061
  • Advanced Studies in Clinical and Experimental Research in GastroenterologyPubmed:26955389
  • Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway.pubmed:27678302
  • Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in ratspubmed:28341485
  • Deletion of MCP-1 Impedes Pathogenesis of Acid Ceramidase DeficiencyPubmed:29379059
  • A critical role of hepatitis B virus polymerase in cirrhosis, hepatocellular carcinoma, and steatosisPubmed:29321963
  • Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituentsPubmed: 28095910
  • Hepatic pathology and altered gene transcription in a murine model of acid ceramidase deficiencyPubmed: 31186526
  • Corticosterone-mediated microglia activation affects dendritic spine plasticity and motor learning functions in minimal hepatic encephalopathyPubmed: 31437533
  • Liraglutide Attenuates Nonalcoholic Fatty Liver Disease by Modulating Gut Microbiota in Rats Administered a High-Fat DietPubmed: 32149099
  • Biological Safety and Biodistribution of Chitosan NanoparticlesPubmed: 32340313
  • Effects of Tempol in Lipopolysaccharide-Induced Liver Injury
  • Antimalarial Effect of the Total Glycosides of the Medicinal Plant, Ranunculus japonicus33925018
  • Taraxasterol mitigates Con A-induced hepatitis in mice by suppressing interleukin-2 expression and its signaling in T lymphocytes34848154
  • NFAT inhibitor 11R-VIVIT ameliorates mouse renal fibrosis after ischemia-reperfusion-induced acute kidney injury34937917
  • Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver …Pubmed:35212074
  • Blockade of mIL‐6R alleviated lipopolysaccharide‐induced systemic inflammatory response syndrome by suppressing NF‐κB‐mediated Ccl2 expression and …Pubmed:35548710
  • Dimethyl fumarate ameliorates autoimmune hepatitis in mice by blocking NLRP3 inflammasome activationPubmed:35605433

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