Multiplex Assay Kit for Asymmetrical Dimethylarginine (ADMA) ,etc. by CBA (Cytometric Bead Array) 
N,N-Dimethylarginine; NG,NG-Dimethylarginine dihydrochloride; Asymmetric Dimethylarginine
Overview
Properties
- Product No.CMB301Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathwayCardiovascular biology
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of Asymmetrical Dimethylarginine (ADMA) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Asymmetrical Dimethylarginine (ADMA) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 93-101 | 98 |
| EDTA plasma(n=5) | 95-103 | 99 |
| heparin plasma(n=5) | 78-101 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Asymmetrical Dimethylarginine (ADMA) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Asymmetrical Dimethylarginine (ADMA) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Asymmetrical Dimethylarginine (ADMA) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 92-101% | 80-103% | 82-93% | 85-99% |
| EDTA plasma(n=5) | 78-99% | 87-101% | 82-98% | 80-90% |
| heparin plasma(n=5) | 81-94% | 89-103% | 81-90% | 82-95% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:ADMA) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Asymmetrical Dimethylarginine (ADMA) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Asymmetrical Dimethylarginine (ADMA) ,etc. by CBA (Cytometric Bead Array) and unlabeled Asymmetrical Dimethylarginine (ADMA) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Asymmetrical Dimethylarginine (ADMA) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Asymmetrical Dimethylarginine (ADMA) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Asymmetrical Dimethylarginine (ADMA) ,etc. by CBA (Cytometric Bead Array) in the sample.
Giveaways
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Citations
- Interactions among Vascular-Tone Modulators Contribute to High Altitude Pulmonary Edema and Augmented Vasoreactivity in HighlandersPubMed: PMC3439466
- Effect of CCL5 on dimethylarginine dimethylaminohydrolase-1 production in vascular smooth muscle cells from spontaneously hypertensive rats.Pubmed: 23834755
- Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats.Pubmed:25475507
- Correlation research on ADMA plasma levels and left ventricular function of peritoneal dialysis patientsPubmed:25550968
- Asymmetric dimethylarginine contributes to airway nitric oxide deficiency in patients with COPDPubMed: 26076870
- Dimethylarginine dimethylaminohydrolase-1 mediates inhibitory effect of interleukin-10 on angiotensin II-induced hypertensive effects in vascular smooth muscle cells of spontaneously hypertensive ratsPubMed: 26375520
- Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in ratsPubMed: 25475507
- Dimethylarginine Dimethylaminohydrolase 2 (DDAH 2) Gene Polymorphism, Asymmetric Dimethylarginine (ADMA) Concentrations, and Risk of Coronary Artery Disease: A Case-Control Studypubmed:27677852
- Increased serum concentrations of asymmetric dimethylarginine (ADMA) in patients with early-onset coronary artery disease.pubmed:27884754
- Asymmetric dimethylarginine (ADMA) elevation and arginase up-regulation contribute toendothelial dysfunction related to insulin resistance in rats and morbidly obese humans.pubmed:26840628
- Dimethylarginine dimethylaminohydrolase-1 (DDAH1) is frequently upregulated in prostate cancer, and its overexpression conveys tumor growth and angiogenesis by metabolizing asymmetric dimethylarginine (ADMA).pubmed:29150732
- A Novel Role of Irbesartan in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats: Targeting DDAH/ADMA and EGFR/ERK SignalingPubmed:29523851
- Multivessel analysis of progressive vascular aging in the rat: Asynchronous vulnerability among vascular territoriesPubmed:29605639
- Arginine bioavailability and endothelin-1 system in the regulation of vascular function of umbilical vein endothelial cells from intrauterine growth restricted newbornsDoi: 10.1016/j.numecd.2018.09.002
- Probucol improves erectile function via Activation of Nrf2 and coordinates the HO-1/DDAH/PPAR-γ/eNOS pathways in streptozotocin-induced diabetic ratsPubmed: 30454888
- Demographic, clinical and lifestyle predictors for severity of erectile dysfunction and biomarkers level in Malaysian patients
- Novel cellularly active inhibitor regresses DDAH1 induced prostate tumor growth by restraining tumor angiogenesis through targeting DDAH1/ADMA/NOS pathwayPubmed: 30673277
- Nebivolol prevents the increase of asymmetric dimethylarginine and oxidants in hyperhomocysteinemic rats
- The Role of Reactive Oxygen Species and Nitric Oxide in the Inhibition of Trichophyton rubrum Growth by HaCaT CellsPubmed: 32104540
- Blueberry anthocyanin‑enriched extract ameliorates transverse aortic constriction‑induced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in …Pubmed: 31746378
- Effect of a Supervised Peridialytic Exercise Program on Serum Asymmetric Dimethylarginine in Maintenance Hemodialysis PatientsPubmed: 33163233
- The Usefulness of Serum Biomarkers in the Early Stages of Diabetic Retinopathy: Results of the EUROCONDOR Clinical TrialPubmed: 32344735
- Levels of endothelial substances in patients with newly identified hypertension compared with healthy controls33463628
- Non-functioning adrenal incidentalomas may increase toxic metabolites34255169
- Effect of a Low Dose of Carvedilol on Cyclophosphamide-Induced Urinary Toxicity in Rats—A Comparison with Mesna34959638
- HIF-1α and Nrf2 regulates hypoxia induced overexpression of DDAH1 through promoter activation in prostate cancerPubmed:35644470