Multiplex Assay Kit for Bcl2 Associated X Protein (Bax) ,etc. by CBA (Cytometric Bead Array) Homo sapiens (Human) Protein, Antibody, Assaykit, Service

BCL2L4; Bax Zeta; Apoptosis regulator BAX; Bcl-2-like protein 4

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 559.00 US$ 798.00
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  • Multiplex Assay Kit for Bcl2 Associated X Protein (Bax) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Bcl2 Associated X Protein (Bax) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Bcl2 Associated X Protein (Bax) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Bcl2 Associated X Protein (Bax) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-104 96
EDTA plasma(n=5) 96-103 101
heparin plasma(n=5) 80-90 84

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2 Associated X Protein (Bax) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2 Associated X Protein (Bax) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bcl2 Associated X Protein (Bax) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-93% 87-104% 85-102% 87-101%
EDTA plasma(n=5) 81-90% 80-96% 80-101% 84-99%
heparin plasma(n=5) 87-97% 86-98% 88-96% 83-91%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Bcl2 Associated X Protein (Bax) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Bcl2 Associated X Protein (Bax) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Bcl2 Associated X Protein (Bax) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Bcl2 Associated X Protein (Bax) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Bcl2 Associated X Protein (Bax) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • Neurotoxic effect of lead on rats: Relationship to ApoptosisNCBI: PMC3883608
  • Effects of hepatocyte growth factor in myocarditis rats induced by immunization with porcine cardiac myosinPubmed: 24327573
  • Cu (II) (PICOLINYL-L-TRYPTOPHAN)2 AS AN EFFECIENT MULTIFUNCTIONAL RADIOPROTECTORAsj-oa: Source
  • Inhibition of small HA fragment activity and stimulation of A2A adenosine receptor pathway limit apoptosis and reduce cartilage damage in experimental arthritisPubmed:25511416
  • The combined treatment with novel platinum(II) complex and anti-MUC1 increases apoptotic response in MDA-MB-231 breast cancer cellsPubMed: 26112902
  • Inhibition of small HA fragment activity and stimulation of A2APubMed: 25511416
  • Hepatoprotective Effects of Rubus idaeus L. Leaves against CCl4-Induced Liver Injury via Antioxidant, Anti-inflammatory and Antiapoptotic Mechanismsopenview:626440
  • In Vivo Characterization of ARN14140, a Memantine/Galantamine-Based Multi-Target Compound for Alzheimer's Diseasepubmed:27609215
  • An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's …articles:journal-of-alzheimers-disease
  • Amyloid toxicity is enhanced after pharmacological or genetic invalidation of the 1 receptor pubmed:29129596
  • Mechanistic insights into the effects of quercetin and/or GLP-1 analogue liraglutide on high-fat diet/streptozotocin-induced type 2 diabetes in ratspubmed:28554128
  • Mechanism of anticancer action of novel berenil complex of platinum(II) combined with anti-MUC1 in MCF-7 breast cancer cells10.3892/ol.2017.7623
  • A polysaccharide (PNPA) from Pleurotus nebrodensis ameliorates hepatic ischemic/reperfusion (I/R) injury in ratspubmed:28709897
  • Potentiation of Apoptosis in Rat Striatum Exposed to Endosulfan and the Role of Quercetin10.1007/s13530-017-0325-1
  • Cardioprotective effect of the xanthones from Gentianella acuta against myocardial ischemia/reperfusion injury in isolated rat heartpubmed:28686977
  • Viscum articulatum Burm. f. aqueous extract exerts antiproliferative effect and induces cell cycle arrest and apoptosis in leukemia cellsPubmed:29555410
  • Mechanism of anticancer action of novel berenil complex of platinum (II) combined with anti-MUC1 in MCF-7 breast cancer cellsPubmed:29434943
  • Association of Bax and Bcl-2 Functional Polymorphisms and Protein Levels with Posttraumatic Stress DisorderpaperID:82598
  • Taxifolin protects rat against myocardial ischemia/reperfusion injury by modulating the mitochondrial apoptosis pathwayPubmed: 30723634
  • INSTITUTO DE CIENCIAS BÁSICAS Y PRECLÍNICAS “VICTORIA DE GIRÓN” DEPARTAMENTO DE HISTOLOGÍA
  • LncRNA SNHG3 promotes clear cell renal cell carcinoma proliferation and migration by upregulating TOP2APubmed: 31505165
  • LCZ696 (sacubitril/valsartan) protects against cyclophosphamide-induced testicular toxicity in rats: Role of neprilysin inhibition and lncRNA TUG1 in ameliorating …Pubmed: 32197949
  • Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cellsPubmed: 33206337
  • Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low− dose of …Pubmed: 32915453
  • Alogliptin attenuates cyclophosphamide-induced nephrotoxicity: a novel therapeutic approach through modulating MAP3K/JNK/SMAD3 signaling cascadePubmed: 32869669
  • Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats
  • Potential effect of adrenomedullin on metabolic and endocrinal dysfunctions in the experimentally induced polycystic ovary: Targeting implication of endoplasmic ¡­33491863
  • Potential Therapeutic Effects of New Ruthenium (III) Complex with Quercetin: Characterization, Structure, Gene Regulation, and Antitumor and Anti-Inflammatory Studies (RuIII/Q Novel Complex Is a Potent Immunoprotective Agent)
  • Allicin ameliorates renal ischemia/reperfusion injury via inhibition of oxidative stress and inflammation in rats34426252
  • Galangin mitigates oxidative stress, inflammation, and apoptosis in a rat model of methotrexate hepatotoxicity34729716
  • Selenium nanoparticles overcomes sorafenib resistance in thioacetamide induced hepatocellular carcinoma in rats by modulation of mTOR, NF-κB pathways and …Pubmed:35640776
  • Piracetam attenuates cyclophosphamide-induced hepatotoxicity in rats: Amelioration of necroptosis, pyroptosis and caspase-dependent apoptosisPubmed:35636581

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