Multiplex Assay Kit for Caspase 7 (CASP7) ,etc. by CBA (Cytometric Bead Array) 
CMH1; ICE-LAP3; MCH3; Caspase 7 Apoptosis-Related Cysteine Peptidase; Cysteine Aspartate Protease 7; Cysteinyl Aspartate Specific Proteinases 7
Overview
Properties
- Product No.CMA449Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & KinaseApoptosis
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Caspase 7 (CASP7) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Caspase 7 (CASP7) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 82-98 | 86 |
| EDTA plasma(n=5) | 78-90 | 82 |
| heparin plasma(n=5) | 87-97 | 91 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 7 (CASP7) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 7 (CASP7) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Caspase 7 (CASP7) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-97% | 93-101% | 92-105% | 78-101% |
| EDTA plasma(n=5) | 92-99% | 84-103% | 88-103% | 93-101% |
| heparin plasma(n=5) | 78-96% | 80-92% | 99-105% | 78-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Caspase 7 (CASP7) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Caspase 7 (CASP7) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Caspase 7 (CASP7) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Caspase 7 (CASP7) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.
Giveaways
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Citations
- Protective Effects of Morus alba Leaves Extract on Ocular Functions of Pups from Diabetic and Hypercholesterolemic Mother RatsPubMed: 21697998
- Analysis of fine structure and biochemical changes of retina during aging of Wistar albino ratsOnlinelibrary: ceo.12123
- Aging-related changes of optic nerve of Wistar albino ratsPubmed:Pmc4039266
- Inhibition of small HA fragment activity and stimulation of A2A adenosine receptor pathway limit apoptosis and reduce cartilage damage in experimental arthritisPubmed:25511416
- Inhibition of small HA fragment activity and stimulation of A2APubMed: 25511416
- Effects of elevated body temperature on structure and function of cerebrum and cerebellum of Wistar male albino ratsPdf-Files: Issue-4-2015
- Role of Pomegranate Juice and Atorvastatin in Ameliorating Spinal Neurotoxicity of Wistar Rats Maternally Fed on Hypercholesterolemic DietDOI: 10.4172/2155-9929.1000316
- Design, synthesis, and screening of amino thiophene carboxamide derivatives on hepatocellular carcinomaas VEGFR-2InhibitorsPubmed: 30191744
- Synergistic effects of pomegranate juice and atorvastatin for improving cerebellar structure and function of breast-feeding rats maternally fed on a high cholesterol dietPubmed: 32339653
- Fish-oil supplementation improves retinal injury induced by diabetes and hypercholesterolemia in male Wistar rats