Multiplex Assay Kit for Catalase (CAT) ,etc. by CBA (Cytometric Bead Array) Mus musculus (Mouse) Protein, Antibody, Assaykit, Service

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 605.00 US$ 864.00
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  • Multiplex Assay Kit for Catalase (CAT) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Catalase (CAT) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Catalase (CAT) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Catalase (CAT) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-99 95
EDTA plasma(n=5) 78-91 81
heparin plasma(n=5) 88-96 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Catalase (CAT) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Catalase (CAT) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Catalase (CAT) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-98% 95-103% 82-91% 83-102%
EDTA plasma(n=5) 91-99% 84-97% 92-105% 95-104%
heparin plasma(n=5) 85-103% 79-95% 98-105% 79-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Catalase (CAT) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Catalase (CAT) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Catalase (CAT) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Catalase (CAT) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Catalase (CAT) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • Proteomic Analysis of Polymorphonuclear Neutrophils Identifies Catalase as a Novel Biomarker of Abdominal Aortic AneurysmAhajournals: source
  • Phloroglucinol protects gastric mucosa against ethanol-induced injury through regulating myeloperoxidase and catalase activitiesPubMed: 20880383
  • Antioxidant profile of salivary glands in high fat diet-induced insulin resistance ratsPubmed: 24106991
  • Iodinated contrast media can induce long-lasting oxidative stress in hemodialysis patients.Pubmed: 24142649
  • Grape seed extract and Zinc containing nutritional food supplement delays onset and progression of Streptozocin-induced diabetic cataract in Wistar ratsSpringer: Source
  • 过氧化氢酶基因 ３８９Ｃ＞Ｔ多态位点在重庆汉族人群中的分布及其与噪音性耳聋的 关联研究Tmmu:Source
  • Genetic polymorphisms (Pro197Leu of Gpx1, +35A/C of SOD1, PubMed: 26674569
  • Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetesPubMed: 26143097
  • Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human salivaPubMed: 26608886
  • КЛИНИЧЕСКОЕ ЗНАЧЕНИЕ ФАКТОРОВ КЛЕТОЧНОЙ РЕГУЛЯЦИИ И ФЕРМЕНТОВ АНТИОКСИДАНТНОЙ СИСТЕМЫ У ПАЦИЕНТОК РЕПРОДУКТИВНОГО ВОЗРАСТА С НАРУЖНЫМ ГЕНИТАЛЬНЫМ ЭНДОМЕТРИdocs:225188
  • Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients.pubmed:27748187
  • Effect of Indole-3-carbinol and/or Metformin on Female Patients with Ulcerative Colitis (Premalignant Condition): Role of Oxidative Stress, Apoptosis and Proinflammatory Cytokinesjcrt:5
  • Orthodontic treatment modifies the oxidant-antioxidant balance in saliva of clinically healthy subjects.pubmed:28242484
  • Laminarin protects against hydrogen peroxide-induced oxidative damage in MRC-5 cells possibly via regulating NRF2pubmed:28785522
  • Evaluation of ADMA, carbonyl groups, CAT and NKA in depressed patients with and without posttraumatic stress disorder.pubmed:28554099
  • Biochemical and molecular alterations in freshwater mollusks as biomarkers for petroleum product, domestic heating oilPubmed:29660615
  • Multi-biomarker responses after exposure to organophosphates chlorpyrifos in the freshwater mussels and snails10.1080:10807039.2018.1460800
  • Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathwayPubmed:30116359
  • A red pumpkin seed extract reduces melanosome transfer to keratinocytes by activation of Nrf2 signalingPubmed:30047218
  • Cholecystectomy Patients with High Plasma Level of Catalase Have Significantly Lower Analgesia Requirement: A Prospective Study of Two Different …Pubmed: 30194197
  • Plasma Catalase in Relation to Pain Following Midline Laparotomy: A Prospective Study of Patients with Benign Diseases and Patients with CancerPubmed: 30396975
  • Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed ratsDoi: 10.1016/j.joim.2018.12.002
  • Protective effects of sulforaphane on diabetic retinopathy: activation of the Nrf2 pathway and inhibition of NLRP3 inflammasome formationPubmed: 30606939
  • An in vitro protocol to study the effect of hyperglycemia on intracellular redox signaling in human retinal pigment epithelial (ARPE-19) cellsPubmed: 30628023
  • Nitrotyrosine (NT), a Nitrosative Stress Biomarker, Plasma Concentrations in Gallstone Disease and Cancer PatientsPubmed: 30711961
  • Rectus Sheath Block (RSB) Analgesia Could Enhance Significantly the Patient Satisfaction Following Midline Laparotomy in Benign Disease and in Cancer: A …Pubmed: 30842172
  • MicroRNA‑21 contributes to the puerarin‑induced cardioprotection via suppression of apoptosis and oxidative stress in a cell model of ischemia/reperfusion injuryPubmed: 31115556
  • Selected elements of extracellular matrix of the skin in diabetes and insulin resistancePubmed: 31146169
  • European Journal of Biomedical AND Pharmaceutical sciences
  • The Rectus Sheath Block (RSB) Analgesia Following Laparotomy Could Affect Malonidialdehyde (MDA) Concentrations in Benign Disease and CancerPubmed: 31892574
  • A Machine Learning-driven Study Indicates Emodin Improves Cardiac Hypertrophy by Modulation of Mitochondrial SIRT3 SignalingPubmed: 32135248
  • Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease DevelopmentPubmed: 32751983
  • AMELIORATIVE EFFECT OF AQUEOUS EXTRACT OF HIBISCUS SABDARIFFA (ROSELLE) ON SALT-INDUCED HYPERTENSION IN WISTAR RATS
  • In Vitro Cell Culture Study of Anticholesterol and Antioxidant Activities of Myrmecodia platytyrae (MyP) Extract in WRL-68 Cells
  • Lutein reverses hyperglycemia-mediated blockage of Nrf2 translocation by modulating the activation of intracellular protein kinases in retinal pigment epithelial (ARPE …
  • Plasma catalase (CAT) levels in gallstone disease: Cholecystectomy patients with high plasma levels of CAT have significantly lower number of analgesic …
  • Effects of Tualang Honey on Pain Behaviour and Oxidative Stress in the Thalamus of Prenatally Stressed Rat Offspring
  • Toxic Trace Metals and Pathological Changes in Organs of Rats Fed with Extract of Polluted Grasses
  • Effects of Ganoderma lucidum polysaccharides on different pathways involved in the development of spinal cord ischemia reperfusion injury: biochemical ¡­33689849
  • Mechanisms of Trx2/ASK1-Mediated Mitochondrial Injury in Pemphigus Vulgaris33763469
  • Attenuation of Oxidative Stress and Inflammatory Response by Chronic Cannabidiol Administration Is Associated with Improved n-6/n-3 PUFA Ratio in the White and?¡­34064937
  • Lycium barbarum polysaccharides attenuate cardiovascular oxidative stress injury by enhancing the Keap1/Nrf2 signaling pathway in exhaustive exercise rats34278476
  • Associations of Antioxidant Enzymes with the Concentration of Fatty Acids in the Blood of Men with Coronary Artery Atherosclerosis34945751
  • Plasma Concentration of the Lipid Peroxidation (LP) Biomarker 4-Ηydroxynonenal (4-HNE) in Benign and Cancer PatientsPubmed:35241533
  • α-Lipoic acid ameliorates inflammation state and oxidative stress by reducing the content of bioactive lipid derivatives in the left ventricle of rats fed a high-fat dietPubmed:35569738
  • Bio-Evaluation of the Wound Healing Activity of Artemisia judaica L. as Part of the Plant's Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory …Pubmed:35204215

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