Multiplex Assay Kit for Chemerin (CHEM) ,etc. by CBA (Cytometric Bead Array) 
TIG2; RARRES2; Tazarotene-Induced Gene 2; Retinoic Acid Receptor Responder 2,Tazarotene Induced; RAR-responsive protein TIG2
Overview
Properties
- Product No.CMA945Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryCytokineMetabolic pathwayInfection immunity
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Chemerin (CHEM) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Chemerin (CHEM) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-104 | 90 |
| EDTA plasma(n=5) | 81-96 | 92 |
| heparin plasma(n=5) | 93-105 | 97 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemerin (CHEM) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemerin (CHEM) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chemerin (CHEM) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-97% | 87-94% | 78-94% | 83-90% |
| EDTA plasma(n=5) | 93-101% | 96-104% | 87-94% | 98-105% |
| heparin plasma(n=5) | 86-101% | 81-93% | 80-94% | 94-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Chemerin (CHEM) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Chemerin (CHEM) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Chemerin (CHEM) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Chemerin (CHEM) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.
Giveaways
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Citations
- Serum chemerin and vaspin in non-alcoholic fatty liver diseasePubMed: 20095887
- Chemerin, vaspin and insulin resistance in chronic hepatitis CWiley: source
- Expression and regulation of chemerin during rat pregnancyScienceDirect: S0143400412000902
- Chronic ethanol consumption increases the levels of chemerin in the serum and adipose tissue of humans and ratsNature: 201211
- Ankilozan spondilitli hastalarda adipositokin düzeyleri ve insülin direnciRaeddergisi: Source
- Association of chemerin with oxidative stress, inflammation and classical adipokines in non-diabetic obese patientsPubmed: 24702860
- Strong correlations between circulating chemerin levels and lipoprotein subfractions in nondiabetic obese and nonobese subjectsPubmed: 24303851
- Impact of OSA on Biological Markers in Morbid Obesity and Metabolic SyndromePubmed:24106991
- Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsiaPubmed:24840719
- Relationship between plasma chemerin levels and disease severity in COPD patientsPubmed:24865134
- Elevated serum chemerin in Chinese women with hyperandrogenic PCOSPubmed:24927079
- Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats.Pubmed:25471281
- Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty RatsPubmed:25697126
- Chemerin and calprotectin levels correlate with disease activity and inflammation markers in psoriasis vulgarisSciencedirect:S1027811714000470
- Serum Chemerin Levels in relation to Osteoporosis and Bone Mineral Density: A Case-Control StudyPubMed: 26170530
- Association of Serum Chemerin Levels with Acute Ischemic Stroke and Carotid Artery Atherosclerosis in a Chinese PopulationPubMed: 26471865
- Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant ratsPubMed: 25471281
- Effects of alpha-lipoic acid on chemerin secretion in 3T3-L1 and human adipocytesPubmed:26721419
- Association of chemerin levels and bone mineral density in Chinese obese postmenopausalwomen.pubmed:27583869
- Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague …Pubmed:29849871
- Chemerin is associated with diabetic retinopathy in type 2 diabetesPubmed:25848840
- Expression of chemerin in the synovial fluid of patients with temporomandibular joint disordersPubmed:29331039
- Epigenetic regulation of Amphiregulin and Epiregulin in colorectal cancerPubmed: 30252132
- The decline of whole-body glucose metabolism in ovariectomized ratsPubmed: 30292771
- Chemerin alleviates acute pancreatitis in the rat thorough modulation of NF-κB signalDoi: 10.1016/j.pan.2019.02.005
- Protective effect of vitamin D supplementation in a rat modal of preeclampsia: a possible implication of chemerinPubmed: 30922121
- Chemerin alleviates acute pancreatitis in the rat thorough modulation of NF-kB signalPubmed: 30833212
- Association between High Levels of Circulating Chemerin and Colorectal Adenoma in MenPubmed: 31269491
- Vitamin D Supplementation Reduces Serum Chemerin Level in Gestational Diabetes Mellitus Rat Model
- Plasma levels of adiponectin and chemerin are associated with early stage of atherosclerosis in older adults with type 2 diabetes mellitus
- The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren33575861
- Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren33834274
- Altered Serum Marker of Adipokines Profile in Breast Cancer Women
- Association of High Serum Chemerin with Bone Mineral Density Loss and Osteoporotic Fracture in Elderly Chinese WomenPubmed:35140527