Multiplex Assay Kit for Chitinase-3-like Protein 1 (CHI3L1) ,etc. by CBA (Cytometric Bead Array) 
GP39; CHI3-L1; YKL40; Chondrex; Chitinase 3-Like 1; Chondrocyte Protein YKL40; 39 kDa synovial protein
Overview
Properties
- Product No.CMB463Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathwayInfection immunityBone metabolismRheumatology
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Chitinase-3-like Protein 1 (CHI3L1) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Chitinase-3-like Protein 1 (CHI3L1) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 96-103 | 99 |
| EDTA plasma(n=5) | 80-103 | 98 |
| heparin plasma(n=5) | 80-98 | 88 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chitinase-3-like Protein 1 (CHI3L1) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chitinase-3-like Protein 1 (CHI3L1) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chitinase-3-like Protein 1 (CHI3L1) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-97% | 93-101% | 92-105% | 91-99% |
| EDTA plasma(n=5) | 84-94% | 81-88% | 85-102% | 88-95% |
| heparin plasma(n=5) | 98-105% | 88-102% | 94-103% | 79-88% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Chitinase-3-like Protein 1 (CHI3L1) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Chitinase-3-like Protein 1 (CHI3L1) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Chitinase-3-like Protein 1 (CHI3L1) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Chitinase-3-like Protein 1 (CHI3L1) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.
Giveaways
Increment services
Citations
- Serum YKL-40: a potential biomarker for psoriasis or endothelial dysfunction in psoriasis?Pubmed:25421412
- YKL-40 is correlated with FEV1 and the asthma control test (ACT) in asthmatic patients: influence of treatmentPubmed:25578181
- Assessment of pulsed electromagnetic field therapy with Serum YKL‐40 and ultrasonography in patients with knee osteoarthritisPubMed: 25955771
- YKL-40 expression in chronic obstructive pulmonary disease: relation to acute exacerbations and airway remodelingPubmed:27013031
- Compositions and Methods for Diagnosing Lung Cancery2016:0077095.html
- Chronic obstructive pulmonary disease (COPD)--from biomarkers to clinical phenotypesISBN:978-951-51-4039-5
- YKL-40 is a local marker for inflammation in patients with pseudoexfoliation syndromePubmed: 30560917
- Astrocytes induce proliferation of oligodendrocyte progenitor cells via connexin 47-mediated activation of Chi3l1 expressionPubmed: 31002152
- Differences in Osteoimmunological Biomarkers Predictive of Psoriatic Arthritis among a Large Italian Cohort of Psoriatic PatientsPubmed: 31717649
- Oligodendrocyte Progenitor Cells Increase Chi3l1 Secretion in Exosome to Activate Myh9 and Promote Proliferation through Connexin47 Connected to …
- High Blood Eosinophil and YKL-40 Levels, as Well as Low CXCL9 Levels, are Associated with Increased Readmission in Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease33814903