Multiplex Assay Kit for Copeptin (CPP) ,etc. by CBA (Cytometric Bead Array) 
C-Terminal Provasopressin; Vasopressin-Neurophysin 2-Copeptin
Overview
Properties
- Product No.CMA365Po
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Copeptin (CPP) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Copeptin (CPP) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 97-105 | 102 |
| EDTA plasma(n=5) | 94-105 | 102 |
| heparin plasma(n=5) | 90-102 | 95 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Copeptin (CPP) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Copeptin (CPP) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Copeptin (CPP) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-92% | 82-102% | 78-95% | 98-105% |
| EDTA plasma(n=5) | 79-92% | 82-102% | 78-103% | 83-97% |
| heparin plasma(n=5) | 98-105% | 79-97% | 80-102% | 79-90% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:CPP) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Copeptin (CPP) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Copeptin (CPP) ,etc. by CBA (Cytometric Bead Array) and unlabeled Copeptin (CPP) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Copeptin (CPP) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Copeptin (CPP) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Copeptin (CPP) ,etc. by CBA (Cytometric Bead Array) in the sample.
Giveaways
Increment services
Citations
- Copeptin: May it be a novel biomarker for insulin theraphy in diabetes? An animal study.Ejmanager: Source
- Serum copeptin in children exposed to maltreatmentPubmed:27278269
- Methods for Diagnosis and Treatment of Concussion or Brain Injury2016:0103140
- Role of enteroendocrine L‐cells in arginine vasopressin‐mediated inhibition of colonic anion secretionPubmed:27037495
- Can hypoxia enhance sexual arousal?-Molecular-biological analysis of the hypothalamus in male rats placed with oestrous female rats under hypoxic …files:ijcem0031358.pdf
- Orthostatic Challenge Shifts the Hemostatic System of Patients Recovered from Stroke toward Hypercoagulability.pubmed:28223937
- N-Terminal Prohormone of Brain Natriuretic Peptide but not C-Terminal Pre-Pro Vasopressin (Copeptin) Level is Associated with the Response to Antihypertensive …pubmed:29190613
- Early Prediction of Preeclampsiajournals:297397
- Evaluation of Urinary Aquaporin 2 and Plasma Copeptin as Biomarkers of Effectiveness of Desmopressin Acetate for the Treatment of Monosymptomatic Nocturnal Enuresis.pubmed:28457803
- Experimental Heat Stress Nephropathy and Liver Injury are Improved by AllopurinolPubmed:29667911
- Angiotensin AT Receptors Expressed in Vasopressin-Producing Cells of the Supraoptic Nucleus Contribute to the Osmotic Control of VasopressinPubmed:29364700
- Rehydration with fructose worsens dehydration-induced renal damagePubmed:30005632
- Copeptin level in the early prediction of cardiorenal syndrome in ratsPubmed:30112047
- 和肽素水平对大鼠心肾综合征的早期预测价值filename:ZBLS201802013
- Abstract P311: Static Urine Osmolality With Elevated First Trimester Urine Copeptin in Human Preeclampsia
- Assessment of serum levels of copeptin and corticotropin-releasing factor in children with monosymptomatic and nonmonosymptomatic nocturnal enuresis
- Cognitive performance is associated with cerebral oxygenation and peripheral oxygen saturation, but not plasma catecholamines, during graded normobaric hypoxia
- Dialysate copeptin and peritoneal transport in incident peritoneal dialysis patientsPubmed: 31187425
- The contribution of collecting duct NOS1 to the concentrating mechanisms in male and female micePubmed: 31241990
- The increase of core temperature affected the progression of kidney injury by repeated heat stress exposurePubmed: 31390229
- Deletion of the serine protease CAP2/Tmprss4 leads to dysregulated renal water handling upon dietary potassium depletionPubmed: 31863073
- Copeptin is independently associated with vascular calcification in chronic kidney disease stage 5Pubmed: 32033584
- Micro RNA 146a gene variant/TNF-α/IL-6/IL-1 β; A cross-link axis inbetween oxidative stress, endothelial dysfunction and neuro-inflammation in acute ischemic stroke …Pubmed: 31738890
- Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with ObesityPubmed: 33027789
- Multimarker Approach to Identify Patients with Coronary Artery Disease at High Risk for Subsequent Cardiac Adverse Events: The Multi-Biomarker StudyPubmed: 32549327
- Relation of Copeptin with Diabetic and Renal Function Markers Among Patients with Diabetes Mellitus Progressing Towards Diabetic NephropathyPubmed: 32505416
- 重组人脑利钠肽治疗慢性肺心病急性加重期的临床效果及对5-HT 浓度的影响
- Validity of different copeptin assays in the differential diagnosis of the polyuria-polydipsia syndrome33980941
- Are the Circulating Levels of Copeptin and Fibronectin Dysregulated in Preeclamptic South African Black Women?
- Copeptin levels are more elevated and associated with cardiovascular events in the unity of chronic ischemic heart and chronic kidney diseases
- High serum copeptin may be a marker of an increased carotid intima-media thickness in asymptomatic patients with type 1 diabetes.
- The Speed of Ingestion of a Sugary Beverage Has an Effect on the Acute Metabolic Response to Fructose
- Dietary K+ acts as a genuine diureticPubmed:34984847
- Spectrally multiplexed assay using gap enhanced nanoparticle for detection of a myocardial infarction biomarker panelPubmed:35190126
- Arcuate NPY is involved in salt‐induced hypertension via modulation of paraventricular vasopressin and brain‐derived neurotrophic factorPubmed:35312067
- Copeptin Concentrations in Plasma of Healthy Neonates in Relation to Water–Electrolyte Homeostasis in the Early Adaptation PeriodPubmed:35327814