Multiplex Assay Kit for Corticotropin Releasing Factor (CRF) ,etc. by CBA (Cytometric Bead Array) 
CRH; Corticotropin Releasing Hormone; Corticoliberin
Overview
Properties
- Product No.CMA835Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryEndocrinologyCardiovascular biologyNeuro scienceHormone metabolism
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Corticotropin Releasing Factor (CRF) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Corticotropin Releasing Factor (CRF) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 91-99 | 94 |
| EDTA plasma(n=5) | 80-97 | 89 |
| heparin plasma(n=5) | 81-93 | 84 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Corticotropin Releasing Factor (CRF) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Corticotropin Releasing Factor (CRF) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Corticotropin Releasing Factor (CRF) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-97% | 80-93% | 90-104% | 97-105% |
| EDTA plasma(n=5) | 82-105% | 89-102% | 80-92% | 99-105% |
| heparin plasma(n=5) | 82-104% | 93-105% | 78-89% | 95-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:CRF) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Corticotropin Releasing Factor (CRF) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Corticotropin Releasing Factor (CRF) ,etc. by CBA (Cytometric Bead Array) and unlabeled Corticotropin Releasing Factor (CRF) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Corticotropin Releasing Factor (CRF) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Corticotropin Releasing Factor (CRF) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Corticotropin Releasing Factor (CRF) ,etc. by CBA (Cytometric Bead Array) in the sample.
Giveaways
Increment services
Citations
- Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male ratsPubmed:24440345
- Immune dysfunction in bipolar disorder and suicide risk: is there an association between peripheral corticotropin‐releasing hormone and interleukin‐1β?Pubmed:24862833
- Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary levelPubmed:25236411
- Acute Stress-Induced Changes in Follicular Dermal Papilla Cells and Mobilization of Mast Cells:Implications for Hair Growth.pubmed:27746640
- Heat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulationpubmed:28663093
- Colonic Transit Time and Gut Peptides in Adult Patients with Slow and Normal Colonic Transit Constipationpubmed:29082242
- Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in naïve and Aβ-treated female ratsPubmed:30028991
- Vaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary …Pubmed:30038568
- Perfluorooctane sulfonate (PFOS) can alter the hypothalamic-pituitary-adrenal (HPA) axis activity by modifying CRF1 and glucocorticoid receptorsPubmed:29807116
- The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic …Pubmed:29379286
- Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine …Pubmed:29540178
- Bioscience Research
- Contribution of DNA methylation in chronic stress–induced cardiac remodeling and arrhythmias in micePubmed: 31431066
- Altered glucocorticoid metabolism represents a feature of macroph‐agingPubmed: 32463582
- Impairment of CRH in the intestinal mucosal epithelial barrier of pregnant Bama miniature pig induced by restraint stress33408312
- O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress33651993