Multiplex Assay Kit for Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc. by CBA (Cytometric Bead Array) 
NTX-I; NTX1
Overview
Properties
- Product No.CMA639Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathwayBone metabolism
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 85-103 | 91 |
| EDTA plasma(n=5) | 85-93 | 90 |
| heparin plasma(n=5) | 78-101 | 95 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 92-101% | 99-105% | 87-95% | 82-93% |
| EDTA plasma(n=5) | 94-102% | 97-105% | 82-101% | 88-105% |
| heparin plasma(n=5) | 87-104% | 89-97% | 87-94% | 87-94% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:NTXI) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc. by CBA (Cytometric Bead Array) and unlabeled Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Cross Linked N-Telopeptide Of Type I Collagen (NTXI) ,etc. by CBA (Cytometric Bead Array) in the sample.
Giveaways
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Citations
- Dyslipidemic high-fat diet affects adversely bone metabolism in mice associated with impaired antioxidant capacityScienceDirect: S0899900709004717
- Differential mRNA expression profiles in proximal tibia of aged rats in response to ovariectomy and low-Ca dietScienceDirect: S875632820800776X
- Promotion of bone formation by naringin in a titanium particle‐induced diabetic murine calvarial osteolysis modelWiley: Source
- A Mediterranean Diet Enriched with Olive Oil Is Associated with Higher Serum Total Osteocalcin Levels in Elderly Men at High Cardiovascular RiskPubMed: PMC3462931
- Biochemical markers of bone resorption are present in human milk: implications for maternal and neonatal bone metabolismPubmed:25109232
- An NMR Metabolomic Study on the Effect of Alendronate in Ovariectomized MicePubmed:25184758
- Strontium Ranelate Reduces the Fracture Incidence in a Growing Mouse Model of Osteogenesis ImperfectaPubMed: 26679066
- Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight womenS1756464617301561
- Impact of a chronic smoking habit on the osteo-immunoinflammatory mediators in the peri-implant fluid of clinically healthy dental implants.pubmed:27328151
- Impact of a triclosan‐containing toothpaste during the progression of experimental peri‐implant mucositis: Clinical parameters and local pattern of osteo …Pubmed:29520826
- Triclosan‐containing fluoride toothpaste on clinical parameters and osteo‐inflammatory mediators when applied in a stent during experimental peri‐implant mucositis …Pubmed: 30659666
- AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activitiesPubmed: 31739209
- Material-Dependent Formation and Degradation of Bone Matrix—Comparison of Two CryogelsPubmed: 32517006
- Comparable Effects of Strontium Ranelate and Alendronate Treatment on Fracture Reduction in a Mouse Model of Osteogenesis Imperfecta33506016
- Effects of icariin on the fracture healing in young and old rats and its mechanism34511043
- Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway34512780