Multiplex Assay Kit for Cyclic Guanosine Monophosphate (cGMP) ,etc. by CBA (Cytometric Bead Array) Pan-species (General) Protein, Antibody, Assaykit, Service

c-GMP; 3',5'-Cyclic GMP; Cyclic 3',5'-GMP; Guanosine 3',5'-Cyclic Phosphate

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  • Multiplex Assay Kit for Cyclic Guanosine Monophosphate (cGMP) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Cyclic Guanosine Monophosphate (cGMP) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of Cyclic Guanosine Monophosphate (cGMP) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Cyclic Guanosine Monophosphate (cGMP) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-105 97
EDTA plasma(n=5) 87-94 90
heparin plasma(n=5) 88-96 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclic Guanosine Monophosphate (cGMP) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclic Guanosine Monophosphate (cGMP) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cyclic Guanosine Monophosphate (cGMP) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-101% 82-90% 79-90% 78-91%
EDTA plasma(n=5) 82-92% 79-103% 78-97% 79-95%
heparin plasma(n=5) 84-98% 80-94% 80-104% 97-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:cGMP) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Cyclic Guanosine Monophosphate (cGMP) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Cyclic Guanosine Monophosphate (cGMP) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Cyclic Guanosine Monophosphate (cGMP) ,etc. by CBA (Cytometric Bead Array) and unlabeled Cyclic Guanosine Monophosphate (cGMP) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Cyclic Guanosine Monophosphate (cGMP) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Cyclic Guanosine Monophosphate (cGMP) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Cyclic Guanosine Monophosphate (cGMP) ,etc. by CBA (Cytometric Bead Array) in the sample.

Giveaways

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Citations

  • Cardioprotective effects of baicalein on heart failure via modulation of Ca2PubMed: 26706290
  • Cardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitroPubmed:26706290
  • Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation09540105.2017.1306494
  • Probucol improves erectile function via Activation of Nrf2 and coordinates the HO-1/DDAH/PPAR-γ/eNOS pathways in streptozotocin-induced diabetic ratsPubmed: 30454888
  • ANP/NPRA Inhibits Epithelial-Mesenchymal Transition of Airway by Targeting Smad3 in Asthma
  • iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine
  • Atrial natriuretic peptide inhibits epithelial-mesenchymal transition (EMT) of bronchial epithelial cells through cGMP/PKG signaling by targeting Smad3 in a murine …Pubmed: 31496319
  • Effect of cGMP-activated aquaporin 1 on TRPV4 in rats with allodynia induced by chronic compression of the dorsal root ganglionPubmed: 31790718
  • Effect of tadalafil on penile nitric oxide synthase and corporal smooth muscle in rats under dutasteride treatmentPubmed: 32160825
  • Epigallocatechin gallate mediated sandwich-like coating for mimicking endothelium with sustained therapeutic nitric oxide generation and heparin releasePubmed: 33143876
  • Antihypertensive effects of allicin on spontaneously hypertensive rats via vasorelaxation and hydrogen sulfide mechanismsPubmed: 32480217
  • The role of adrenergic and muscarinic receptors in stress-induced cardiac injury34245378
  • A nitric oxide-eluting and REDV peptide-conjugated coating promotes vascular healingPubmed:35366606

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