Multiplex Assay Kit for D-Dimer (D2D) ,etc. by CBA (Cytometric Bead Array) 
D 2 Dimer
Overview
Properties
- Product No.CMA506Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryHematologyCardiovascular biology
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant D-Dimer (D2D) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of D-Dimer (D2D) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 94-104 | 101 |
| EDTA plasma(n=5) | 78-94 | 83 |
| heparin plasma(n=5) | 92-99 | 95 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level D-Dimer (D2D) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level D-Dimer (D2D) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of D-Dimer (D2D) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-101% | 83-101% | 84-101% | 79-96% |
| EDTA plasma(n=5) | 81-88% | 89-103% | 89-103% | 97-105% |
| heparin plasma(n=5) | 88-97% | 91-104% | 81-99% | 84-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:D2D) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-D-Dimer (D2D) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled D-Dimer (D2D) ,etc. by CBA (Cytometric Bead Array) and unlabeled D-Dimer (D2D) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to D-Dimer (D2D) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of D-Dimer (D2D) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of D-Dimer (D2D) ,etc. by CBA (Cytometric Bead Array) in the sample.
Giveaways
Increment services
Citations
- Determinants of the thrombogenic potential of multiwalled carbon nanotubesScienceDirect: S0142961211004819
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- Protective Effect of Curcumin on Pulmonary and Cardiovascular Effects Induced by Repeated Exposure to Diesel Exhaust Particles in MicePlosOne: Source
- Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central IndiaPubmed: 23992124
- Assessment of coagulopathy, endothelial injury, and inflammation after traumatic brain injury and hemorrhage in a porcine modelLww:Source
- Protein S exacerbates alcoholic hepatitis by stimulating liver natural killer T cellsPubmed:25399514
- Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous CrisisPubmed:25448261
- Impact of β-Adrenoceptor Blockade on Systemic Inflammation and Coagulation Disturbances in Rats with Acute Traumatic CoagulopathyPubmed:25676919
- Intraperitoneal administration of activated protein C prevents postsurgical adhesion band formation.PubMed: 25575539
- Procoagulant and Fibrinolytic Activity after Polytrauma in RatPubMed: 26632604
- Upregulation of canonical transient receptor potential channel in the pulmonary arterial smooth muscle of a chronic thromboembolic pulmonary hypertension rat modelPubMed: 26155749
- Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptorsPubMed: 25546502
- KLF2 and KLF4 control endothelial identity and vascular integrity.pubmed:28239661
- Activated protein C drives the hyperfibrinolysis of acute traumatic coagulopathypubmed:27841821
- Activated TAFI Promotes the Development of Chronic Thromboembolic Pulmonary Hypertension: A Possible Novel Therapeutic Targetpubmed:28289017
- Inhibition of Cell Apoptosis and Amelioration of Pulmonary Fibrosis by Thrombomodulinpubmed:28739343
- Low dose of alcohol attenuates pro-atherosclerotic activity of thrombin.pubmed:28923781
- Lactadherin Promotes Microvesicle Clearance to Prevent Coagulopathy and Improves Survival of Severe TBI Micepubmed:29162596
- Guided longer pulses from a diagnostic ultrasound and intraclot microbubble enhanced catheter-directed thrombolysis in vivo.pubmed:28417266
- Experimental Evaluation of a New Tissue Factor-Based Topical Hemostat (TT-173) for Treatment of Hepatic BleedingPubmed: 30380352
- The Inhibitory Effect of Protamine on Platelets is Attenuated by Heparin without Inducing Thrombocytopenia in RodentsPubmed: 31533230
- Complement factor D is linked to platelet activation in human and rodent sepsis34396466
- NLRP3 Inflammasome contributes to endotoxin-induced coagulationPubmed:35421682
- Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosisPubmed:35126715