Multiplex Assay Kit for Diacylglycerol (DAG) ,etc. by CBA (Cytometric Bead Array) Pan-species (General) Protein, Antibody, Assaykit, Service

DG; Diglyceride

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 685.00 US$ 979.00
  • Quantity
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  • Multiplex Assay Kit for Diacylglycerol (DAG) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Diacylglycerol (DAG) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of Diacylglycerol (DAG) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Diacylglycerol (DAG) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-99 93
EDTA plasma(n=5) 99-105 102
heparin plasma(n=5) 79-104 87

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Diacylglycerol (DAG) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Diacylglycerol (DAG) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Diacylglycerol (DAG) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-102% 95-105% 84-91% 95-104%
EDTA plasma(n=5) 94-103% 87-95% 96-105% 79-103%
heparin plasma(n=5) 80-93% 99-105% 78-101% 92-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:DAG) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Diacylglycerol (DAG) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Diacylglycerol (DAG) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Diacylglycerol (DAG) ,etc. by CBA (Cytometric Bead Array) and unlabeled Diacylglycerol (DAG) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Diacylglycerol (DAG) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Diacylglycerol (DAG) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Diacylglycerol (DAG) ,etc. by CBA (Cytometric Bead Array) in the sample.

Giveaways

Increment services

Citations

  • Wogonin ameliorates lipotoxicity-induced apoptosis of cultured vascular smooth muscle cells via interfering with DAG-PKC pathwayNature: 2011120a
  • Maternal stress predicts altered biogenesis and the profile of mitochondrial proteins in the frontal cortex and hippocampus of adult offspring ratsPubMed: 26143539
  • Aryl Hydrocarbon Receptor Plays Protective Roles against High Fat Diet (HFD)-induced Hepatic Steatosis and the Subsequent Lipotoxicity via Direct Transcriptional ReCavia (Guinea pig )lation of Socs3 Gene ExpressionPubmed:26865635
  • Pigment Epithelium-Derived Factor (PEDF) Improves Ischemic Cardiac Functional Reserve Through Decreasing Hypoxic Cardiomyocyte Contractility Through PEDF Receptor (PEDF-R).pubmed:27413044
  • PEDF protects cardiomyocytes by promoting FUNDC1‑mediated mitophagy via PEDF-R under hypoxic conditionPubmed:29512692
  • Bouchardatine analogue alleviates NAFLD/NASH in high fat fed mice via blunting ATP synthase activityPubmed: 31113010
  • Gut Akkermansia muciniphila ameliorates non-alcoholic fatty liver disease by L-aspartate via interaction with liver
  • The expression of diacylglycerol kinase isoforms α and ζ correlates with the progression of experimental autoimmune encephalomyelitis in rats34312706

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