Multiplex Assay Kit for Endothelin 1 (EDN1) ,etc. by CBA (Cytometric Bead Array) 
ET1; PPET1; Preproendothelin-1; Big endothelin-1
Overview
Properties
- Product No.CMA482Gu
- Organism SpeciesCavia (Guinea pig ) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryEndocrinologyCardiovascular biology
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Endothelin 1 (EDN1) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Endothelin 1 (EDN1) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 85-92 | 88 |
| EDTA plasma(n=5) | 98-105 | 102 |
| heparin plasma(n=5) | 96-103 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endothelin 1 (EDN1) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endothelin 1 (EDN1) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Endothelin 1 (EDN1) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-104% | 91-99% | 78-94% | 93-104% |
| EDTA plasma(n=5) | 94-101% | 83-97% | 78-94% | 97-105% |
| heparin plasma(n=5) | 99-105% | 96-105% | 81-105% | 78-95% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:EDN1) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Endothelin 1 (EDN1) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Endothelin 1 (EDN1) ,etc. by CBA (Cytometric Bead Array) and unlabeled Endothelin 1 (EDN1) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Endothelin 1 (EDN1) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Endothelin 1 (EDN1) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Endothelin 1 (EDN1) ,etc. by CBA (Cytometric Bead Array) in the sample.
Giveaways
Increment services
Citations
- B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sicknessAPS: source
- CD98 Positive Eosinophils Contribute to T Helper 1 Pattern InflammationPlosOne: Source
- Megakaryocytic leukemia 1 (MKL1) ties the epigenetic machinery to hypoxia-induced transactivation of endothelin-1PubMed: PMC3695508
- Antifibrotic effects of crocetin in scleroderma fibroblasts and in bleomycin-induced sclerotic micePubmed: 24212843
- Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat modelPubmed:25476829
- One or 4 h of “in‐house” reconditioning by machine perfusion after cold storage improve reperfusion parameters in porcine kidneysPubmed:24977654
- The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disordersPubmed:25245337
- Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal InstillationPubmed:25580880
- Pivotal role of mast cell carboxypeptidase A in mediating protection against small intestinal ischemia–reperfusion injury in rats after ischemic preconditioningSciencedirect:S0022480414004909
- The promising effect of barberry (Zereshk) extract against experimental pulmonary microvascular remodeling and hypertension: A comparison with sildenafilPubMed: 26023989
- The effect of high altitude on endothelial and vascular dysfunction markers in preeclamptic patientsPubMed: 26690031
- Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal InstillationPubMed: 25580880
- An in vivo and in vitro study High-dosage Danshen injection induces peripheral vascular endothelial cells injuryPubMed: 26078283
- Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndromescience:article
- Cardiovascular Risk Factors and Markers of Atherosclerosis in Stable Kidney TransplantRecipients.pubmed:27496444
- Association of endothelial dysfunction and cytotoxin-associated gene A-positive Helicobacter pylori in patients with cardiac syndrome X.pubmed:27884380
- Comparative studies on lens structure and function of newborn rats maternally treated with aspartame, monosodium glutamate and galactosepdfcopy:42552
- Abnormal Retinal Structure and Function of Mother Wistar Rats Supplemented Aspartame, Glutamate and Galactose587e5b6508aed3826af45e2d.pdf
- Antioxidant and anti-inflammatory activities of a commercial noni juice revealed by carrageenan-induced paw edemapubmed:27760029
- Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndromearticle:S1872-9312(16)30085-0
- Biochemical and Molecular Markers of Congenital and Senile Cataractous LensesDOI: 10.4172/2155-9929.1000318
- A Study of Effect of Experimental Pre-Eclampsia on Plasma Lipocalin-2 Level in Rats.AN=123819331
- Antihypertensive effects of Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation10.1016/j.jff.2017.05.033
- Selective Insulin-like Growth Factor Resistance Associated with Heart Hemorrhages and Poor Prognosis in a Novel Preclinical Model of the Hematopoietic Acute …Pubmed:29809108
- Isolated kidney perfusion: the influence of pulsatile flowPubmed:29301417
- Endothelin-1 (ET-1), N-terminal fragment of proatrial natriuretic peptide (NTpro-ANP) and tumor necrosis factor alpha (TNF-α) in children with primary hypertension …Pubmed: 30359461
- Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndromePubmed: 30342783
- Short‐Term Isocaloric Intake of a Fructose‐but not Glucose‐Rich Diet Affects Bacterial Endotoxin Concentrations and Markers of Metabolic Health in Normal Weight …Pubmed: 30570214
- Whitening effects of cosmetic formulation in the vascular component of skin pigmentationPubmed: 31074159
- APASL Single Topic Conference DELTA HEPATITIS, 27-28 June 2019, Baku, Azerbaijan
- Potent anti-inflammatory activity of polysaccharides extracted from Blidingia minima and their effect in a mouse model of inflammatory bowel disease
- Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in ratsPubmed: 32616735
- Tianma Gouteng Decoction Exerts Cardiovascular Protection by Upregulating OPG and TRAIL in Spontaneously Hypertensive RatsPubmed: 33133215
- Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency34119534
- Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti …34736077
- Bai-Hu-Tang regulates endothelin-1 and its signalling pathway in vascular endothelial cells34752899
- Post-stroke treatment of storax improves long-term outcomes of stroke in rats34343648
- Laser Doppler flowmetry in assessing the effect of liraglutide on microcirculation in rats with absolute insulin deficiency