Multiplex Assay Kit for Fatty Acid Binding Protein 1 (FABP1) ,etc. by CBA (Cytometric Bead Array) 
FABP-1; FABPL; L-FABP; LFABP; Liver-type fatty acid-binding protein; Fatty Acid Binding Protein 1, Liver
Overview
Properties
- Product No.CMB566Po
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathway
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Fatty Acid Binding Protein 1 (FABP1) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Fatty Acid Binding Protein 1 (FABP1) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 97-104 | 101 |
| EDTA plasma(n=5) | 79-94 | 82 |
| heparin plasma(n=5) | 93-101 | 97 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Binding Protein 1 (FABP1) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Binding Protein 1 (FABP1) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fatty Acid Binding Protein 1 (FABP1) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-103% | 86-104% | 86-97% | 89-105% |
| EDTA plasma(n=5) | 81-101% | 95-103% | 82-97% | 79-103% |
| heparin plasma(n=5) | 83-90% | 97-105% | 95-103% | 93-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Fatty Acid Binding Protein 1 (FABP1) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Fatty Acid Binding Protein 1 (FABP1) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Fatty Acid Binding Protein 1 (FABP1) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Fatty Acid Binding Protein 1 (FABP1) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.
Giveaways
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Citations
- Acute Kidney Injury after Using Contrast during Cardiac Catheterization in Children with Heart DiseasePubmed:25120320
- Ex vivo use of a Rho-kinase inhibitor during renal preservation improves graft function upon reperfusionPubmed:25555715
- Controlled Rewarming after Hypothermia: Adding a New Principle to Renal PreservationPubMed: 26053383
- The role of serum I-FABP concentration in assessment of small intestine mucosa among HIV-infected patientsContent: Early
- Kidney transplantation after oxygenated machine perfusion preservation with Custodiol‐N solutionPubMed: 25882869
- Prediction of renal function upon reperfusion by ex situ controlled oxygenated rewarmingpubmed:27718228
- The Importance of Liver-Fatty Acid Binding Protein in Diagnosis of Liver Damage in Patients with Acute Hepatitispubmed:28571184
- Plasma Free Fatty Acids and their Binding Proteins in Preterm InfantsPubmed:30045009
- Isolated kidney perfusion: the influence of pulsatile flowPubmed:29301417
- Circulating fatty acid-binding protein 1 (FABP1) and nonalcoholic fatty liver disease in patients with type 2 diabetes mellitusPubmed: 32038102
- FABP1 and FABP2 as markers of diabetic nephropathyPubmed: 32922200
- Profile of Intestinal Barrier Functional Markers in Italian Patients with Diarrhea-Predominant IBS: Preliminary Data from a Low-FODMAPs diet Trial
- The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males34071378
- TCA Cycle and Fatty Acids Oxidation Reflect Early Cardiorenal Damage in Normoalbuminuric Subjects with Controlled Hypertension34356333
- Association of plasma fatty acid-binding protein 3 with estimated glomerular filtration rate in patients with type 2 diabetes mellitus34975301