Multiplex Assay Kit for Fatty Acid Binding Protein 4 (FABP4) ,etc. by CBA (Cytometric Bead Array) 
A-FABP; AFABP; AP2; Fatty Acid Binding Protein 4, Adipocyte; Adipocyte Protein 2; Adipocyte-type fatty acid-binding protein
- UOM
- FOB US$ 342.00 US$ 355.00 US$ 375.00 US$ 401.00 US$ 428.00 US$ 467.00 US$ 526.00 US$ 658.00
- Quantity
Overview
Properties
- Product No.CMB693Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathwayEndocrinologyCardiovascular biologyHepatology
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Fatty Acid Binding Protein 4 (FABP4) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Fatty Acid Binding Protein 4 (FABP4) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 97-105 | 101 |
| EDTA plasma(n=5) | 79-94 | 81 |
| heparin plasma(n=5) | 88-98 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Binding Protein 4 (FABP4) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Binding Protein 4 (FABP4) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fatty Acid Binding Protein 4 (FABP4) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-105% | 91-101% | 94-102% | 89-97% |
| EDTA plasma(n=5) | 96-105% | 95-102% | 78-94% | 80-93% |
| heparin plasma(n=5) | 98-105% | 82-95% | 80-96% | 98-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Fatty Acid Binding Protein 4 (FABP4) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Fatty Acid Binding Protein 4 (FABP4) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Fatty Acid Binding Protein 4 (FABP4) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Fatty Acid Binding Protein 4 (FABP4) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.
Giveaways
Increment services
Citations
- Enhanced A-FABP expression in visceral fat: potential contributor to the progression of NASHPubMed: 23091808
- Siesta is associated with reduced systolic blood pressure level and decreased prevalence of hypertension in older adultsPubMed: 26134622
- Overexpression of the A-FABP gene facilitates intermuscular fat deposition in transgenic micePubMed: 25867423
- Cranberries (Oxycoccus quadripetalus) inhibit lipid metabolism and modulate leptin and adiponectin secretion in 3T3-L1 adipocytesPubMed: 25952883
- Increased expression of fatty acid binding protein 4 in preeclamptic Placenta and its relevance to preeclampsiaPubmed:26992681
- Valproate acid (VPA)-induced dysmetabolic function in clinical and animal studies.pubmed:28161274
- Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague …Pubmed:29849871
- Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humansPubmed:29335416
- 25-HC promotes hepatocellular carcinoma metastasis through up-regulation of TLR4 dependent FABP4Pubmed: 31720079
- Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver …Pubmed:35212074
- The Effect of Mineralocorticoid Receptor 3 Antagonists on Anti-Inflammatory and Anti-Fatty Acid Transport Profile in Patients with Heart FailurePubmed:35455943
- FABP4 secreted by M1-polarized macrophages promotes synovitis and angiogenesis to exacerbate rheumatoid arthritisPubmed:35729106