Multiplex Assay Kit for Fibroblast Growth Factor 21 (FGF21) ,etc. by CBA (Cytometric Bead Array) Homo sapiens (Human) Protein, Antibody, Assaykit, Service

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 420.00 US$ 600.00
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  • Multiplex Assay Kit for Fibroblast Growth Factor 21 (FGF21) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Fibroblast Growth Factor 21 (FGF21) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Fibroblast Growth Factor 21 (FGF21) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibroblast Growth Factor 21 (FGF21) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-94 89
EDTA plasma(n=5) 98-105 102
heparin plasma(n=5) 79-104 86

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 21 (FGF21) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 21 (FGF21) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibroblast Growth Factor 21 (FGF21) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-101% 99-105% 99-105% 98-105%
EDTA plasma(n=5) 80-99% 92-105% 98-105% 97-104%
heparin plasma(n=5) 80-99% 78-101% 85-101% 91-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Fibroblast Growth Factor 21 (FGF21) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Fibroblast Growth Factor 21 (FGF21) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Fibroblast Growth Factor 21 (FGF21) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Fibroblast Growth Factor 21 (FGF21) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Fibroblast Growth Factor 21 (FGF21) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • Dietary moderately oxidized oil induces expression of fibroblast growth factor 21 in the liver of pigsLipidworld: source
  • Orphan nuclear receptor Nur77 mediates fasting-induced hepatic fibroblast growth factor 21 expressionEndocrine:Source
  • Alpha lipoic acid induces hepatic fibroblast growth factor 21 expression via up-regulation of CREBHPubmed:25449271
  • Skeletal muscle increases FGF21 expression in mitochondrial disorders to compensate for energy metabolic insufficiency by activating the mTOR–YY1–PGC1α pathwayPubMed: 25843656
  • Associations between FGF21, osteonectin and bone turnover markers in type 2 diabetic patients with albuminuria.pubmed:27916484
  • Association of Bone Metabolic Markers With Diabetic Retinopathy and Diabetic Macular Edema in Elderly Chinese Individuals With Type 2 Diabetes Mellituspubmed:29078839
  • Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21Pubmed:29593555
  • Некоторые аспекты транспортной системы метаболизма железа в зависимости от степени синдрома перегрузки железом у детей с хроническим …
  • Fibroblast growth factor 21 (FGF21) in children and adolescents with chronic kidney disease.Pubmed: 32469231
  • 2 型糖尿病患者血清FGF21, 骨连结蛋白和骨转换指标的相关性研究.
  • Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats33833309
  • The Speed of Ingestion of a Sugary Beverage Has an Effect on the Acute Metabolic Response to Fructose
  • Association of serum fibroblast growth factor 21 with kidney function in a population-based Chinese cohort34918690
  • Oxidative stress-induced growth inhibitor 1 in alcohol-induced liver cirrhosis34969228
  • Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver …Pubmed:35212074
  • Fibroblast growth factor 21, a stress regulator, inhibits Drp1 activation to alleviate skeletal muscle ischemia/reperfusion injuryPubmed:35488034
  • Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling …Pubmed:35585377
  • High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients

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