Multiplex Assay Kit for Fibulin 3 (FBLN3) ,etc. by CBA (Cytometric Bead Array) Mus musculus (Mouse) Protein, Antibody, Assaykit, Service

EFEMP1; DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; EGF-Containing Fibulin-Like Extracellular Matrix Protein 1; Fibrillin-Like; Extracellular protein S1-5

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 605.00 US$ 864.00
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  • Multiplex Assay Kit for Fibulin 3 (FBLN3) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Fibulin 3 (FBLN3) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Fibulin 3 (FBLN3) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibulin 3 (FBLN3) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-101 94
EDTA plasma(n=5) 80-91 80
heparin plasma(n=5) 85-99 89

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibulin 3 (FBLN3) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibulin 3 (FBLN3) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibulin 3 (FBLN3) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-101% 92-99% 94-101% 87-98%
EDTA plasma(n=5) 79-98% 86-94% 88-102% 79-89%
heparin plasma(n=5) 89-102% 86-97% 78-101% 86-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Fibulin 3 (FBLN3) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Fibulin 3 (FBLN3) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Fibulin 3 (FBLN3) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Fibulin 3 (FBLN3) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Fibulin 3 (FBLN3) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • Fibulin-3 as a Blood and Effusion Biomarker for Pleural MesotheliomaPubMed: PMC3761217
  • YKL-40 and mesothelin in the blood of patients with malignant mesothelioma, lung cancer and asbestosis.Pubmed: 24324091
  • Role of fibulin-3 in lung cancer: In vivo and in vitro analysesSpandidos-publications: Source
  • Discovery of new biomarkers for malignant mesotheliomaSpringer:Source
  • DIAGNOSIS OF CELL PROLIFERATIVE DISEASESFreepatentsonline:Source
  • Comparison of fibulin-3 and mesothelin as markers in malignant mesotheliomaPubmed:Pmc4174124
  • Value of plasma and pleural effusion fibulin-3 levels in the diagnosis of malignant pleural mesothelioma effusionsPubmed:23050525
  • Fibulin-3 as a biomarker of response to treatment in malignant mesotheliomaPubMed: 26401134
  • Fibulin-3 levels in malignant pleural mesothelioma are associated with prognosis but not diagnosisPubMed: 26263483
  • Plasma Biomarker Enrichment of Clinical Prognostic Indices in Malignant Pleural Mesothelioma.Pubmed:26903362
  • Diagnostic and Prognostic Biomarkers in the Rational Assessment of Mesothelioma (DIAPHRAGM) study: protocol of a prospective, multicentre, observational study pubmed:27884852
  • HMGB1 and its hyperacetylated isoform are sensitive and specific serum biomarkers to detect asbestos exposure and to identify mesothelioma patientspubmed:26733616
  • Diagnostic and Prognostic Biomarkers in the Rational Assessment of Mesothelioma (DIAPHRAGM) study: protocol of a prospective, multicentre, observational …e013324.abstract
  • Comparison of the Diagnostic Performance of Fibulin-3 and Mesothelin in Patients with Pleural Effusions from Malignant Mesothelioma.pubmed:28314308
  • A Novel Panel of Serum Biomarkers for MPM Diagnosis.pubmed:28348450
  • Association of fibulin-3 concentrations with the presence and severity of knee osteoarthritis: A cross-sectional studypubmed:29195846
  • Plasma Fibulin-3 as a Potential Biomarker for Patients with Asbestos-Related Diseases in the Han Populationpubmed:29200597
  • Placental protein 14 as a potential biomarker for diagnosis of preterm premature rupture of membranesPubmed:29749501
  • The matrix protein Fibulin-3 promotes KISS1R induced triple negative breast cancer cell invasionPubmed:30046386
  • Fibulin-3 and other cartilage metabolism biomarkers in relationship to calprotectin (MRP8/14) and disease activity in rheumatoid arthritis patients treated with …68362.pdf
  • Prediction of pneumoconiosis by serum and urinary biomarkers in workers exposed to asbestos-contaminated mineralsPubmed: 30946771

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