Multiplex Assay Kit for Follistatin Like Protein 1 (FSTL1) ,etc. by CBA (Cytometric Bead Array) 
FRP; FSL1; Follistatin-related protein 1
Overview
Properties
- Product No.CMJ085Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryReproductive science
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Follistatin Like Protein 1 (FSTL1) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Follistatin Like Protein 1 (FSTL1) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 93-101 | 97 |
| EDTA plasma(n=5) | 81-92 | 86 |
| heparin plasma(n=5) | 98-105 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Follistatin Like Protein 1 (FSTL1) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Follistatin Like Protein 1 (FSTL1) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Follistatin Like Protein 1 (FSTL1) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-101% | 86-103% | 87-101% | 80-98% |
| EDTA plasma(n=5) | 89-96% | 92-105% | 95-104% | 81-96% |
| heparin plasma(n=5) | 95-105% | 85-102% | 87-103% | 93-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Follistatin Like Protein 1 (FSTL1) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Follistatin Like Protein 1 (FSTL1) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Follistatin Like Protein 1 (FSTL1) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Follistatin Like Protein 1 (FSTL1) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.
Giveaways
Increment services
Citations
- Serum S100A8/A9, but not follistatin-like protein 1 and interleukin 18, may be a useful biomarker of disease activity in adult-onset Still's disease.PubMed: 22660800
- Regulation of follistatin-like protein 1 expression and secretion in primary human skeletal muscle cellsPubmed: 23419164
- Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferationPubmed:25118038
- Growth Differentiation Factor-15 (GDF-15) Levels Are Associated with Cardiac and Renal Injury in Patients Undergoing Coronary Artery Bypass Grafting with Cardiopulmonary BypassPubmed:25171167
- Growth hormone replacement therapy regulates microRNA-29a and targets involved in insulin resistancePubMed: 26199111
- Association of Circulating Follistatin-Like 1 Levels with Inflammatory and Oxidative Stress Markers in Healthy Menpmc:PMC4856269
- Follistatin like 1 Regulates Hypertrophy in Heart Failure with Preserved Ejection Fraction.pubmed:27430031
- Follistatin like-1 aggravates silica-induced mouse lung injuryPMC5428474
- Plasma follistatin-like protein 1 is correlated with disease severity in patients with acute pulmonary embolism and predicts short-term mortalityISSN:1936-2625
- High circulating follistatin-like protein 1 as a biomarker of a metabolically unhealthy statePubmed: 30745500
- Associations among circulating levels of follistatin-like 1, clinical parameters, and cardiovascular events in patients undergoing elective percutaneous …Pubmed: 31034503
- Improved early detection of ovarian cancer using longitudinal multimarker modelsPubmed: 31937926
- Targeting Follistatin like 1 ameliorates liver fibrosis induced by carbon tetrachloride through TGF-β1-miR29a in micePubmed: 32933544
- Effects of acute endurance exercise on follistatin-like 1 and apelin in the circulation and metabolic organs in ratsPubmed: 32412800
- Prognostic impact of transcardiac gradient of follistatin-like 1 reflecting hemodynamics in patients with dilated cardiomyopathy34334268
- Association between Plasma Follistatin-like Protein 1 Levels and the Presence and Severity of Coronary Artery Disease34853217