Multiplex Assay Kit for Gamma-Aminobutyric Acid (gABA) ,etc. by CBA (Cytometric Bead Array) 
4-Aminobutyric Acid
Overview
Properties
- Product No.CMA900Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategorySignal transductionCardiovascular biologyNeuro scienceBone metabolism
Sign into your account
Share a new citation as an author
Upload your experimental result
Review
Contact us
Please fill in the blank.
-
Packages (Simulation)
-
Packages (Simulation)
-
ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of Gamma-Aminobutyric Acid (gABA) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Gamma-Aminobutyric Acid (gABA) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 84-101 | 92 |
| EDTA plasma(n=5) | 96-105 | 101 |
| heparin plasma(n=5) | 81-95 | 92 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gamma-Aminobutyric Acid (gABA) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gamma-Aminobutyric Acid (gABA) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gamma-Aminobutyric Acid (gABA) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 92-101% | 88-99% | 78-101% | 78-91% |
| EDTA plasma(n=5) | 79-101% | 85-101% | 83-104% | 91-98% |
| heparin plasma(n=5) | 95-105% | 79-98% | 79-105% | 90-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:gABA) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Gamma-Aminobutyric Acid (gABA) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Gamma-Aminobutyric Acid (gABA) ,etc. by CBA (Cytometric Bead Array) and unlabeled Gamma-Aminobutyric Acid (gABA) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Gamma-Aminobutyric Acid (gABA) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Gamma-Aminobutyric Acid (gABA) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Gamma-Aminobutyric Acid (gABA) ,etc. by CBA (Cytometric Bead Array) in the sample.
Giveaways
Increment services
Citations
- Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellumPubMed: 21444355
- Expression of the neuron-specific potassium chloride cotransporter KCC2 in adult rat cochlear.PubMed: 18577424
- Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with AutismPubMed: PMC3623607
- Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic ChildrenPubMed: PMC3694825
- Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD)PubMed: PMC3762604
- Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertensionpubmed:27917928
- Sex differences in the glutamate signaling pathway in juvenile ratsPubmed:28861894
- A novel homozygous mutation in GAD1 gene described in a schizophrenic patient impairs activity and dimerization of GAD67 enzymePubmed: 30341396
- Aucubin Alleviates Seizures Activity in Li-Pilocarpine-Induced Epileptic Mice: Involvement of Inhibition of Neuroinflammation and Regulation of NeurotransmissionPubmed: 30666488
- Differentiation of human glioblastoma U87 cells into cholinergic neuronPubmed: 30928478
- Glutamate Signaling Defects in Propionic Acid Orally Administered to Juvenile Rats as an Experimental Animal Model of Autism
- Effect of gamma radiation on combination therapy of certain antiepileptic drugs in rats.
- Antiepileptic effects of exogenous β‑hydroxybutyrate on kainic acid‑induced epilepsyPubmed: 33101467
- Dementia model mice exhibited improvements of neuropsychiatric symptoms as well as cognitive dysfunction with neural cell transplantation33828024
- Age-dependent neurological phenotypes in a mouse model of PRRT2-related diseases34101060
- Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission34475508
- Adaptogenic potential of ginsenosides against domoic acid‐induced toxicity by regulating neuronal stress and kinate receptors: Ex vivo and in silico studiesPubmed:35102569