Multiplex Assay Kit for Ghrelin (GHRL) ,etc. by CBA (Cytometric Bead Array) 
MTLRP; Growth Hormone-Releasing Peptide
Overview
Properties
- Product No.CMA991Bo
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategorySignal transductionMetabolic pathwayEndocrinologyGastroenterologyHormone metabolism
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Ghrelin (GHRL) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Ghrelin (GHRL) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 87-101 | 92 |
| EDTA plasma(n=5) | 84-97 | 90 |
| heparin plasma(n=5) | 81-98 | 95 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ghrelin (GHRL) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ghrelin (GHRL) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ghrelin (GHRL) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-104% | 97-105% | 89-103% | 83-96% |
| EDTA plasma(n=5) | 83-101% | 97-105% | 82-93% | 80-95% |
| heparin plasma(n=5) | 98-105% | 91-102% | 80-102% | 91-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:GHRL) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Ghrelin (GHRL) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Ghrelin (GHRL) ,etc. by CBA (Cytometric Bead Array) and unlabeled Ghrelin (GHRL) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Ghrelin (GHRL) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Ghrelin (GHRL) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Ghrelin (GHRL) ,etc. by CBA (Cytometric Bead Array) in the sample.
Giveaways
Increment services
Citations
- Expression of ghrelin in human salivary glands and its levels in saliva and serum in Chinese obese children and adolescentsPubMed: 21075359
- Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cowsScienceDirect: S0196978113000533
- Postnatal administration of 2-oxoglutaric acid improves articular and growth plate cartilages and bone tissue morphology in pigs prenatally treated with dexamethasonePubMed: 23211309
- The modifying effects of fish oil on fasting ghrelin mRNA expression in weaned ratsPubMed: 22842192
- Changes in Ghrelin and Obestatin Levels before and after a Meal in Children with Simple Obesity and AnorexiaPubmed: 23774845
- Gastric supply manipulation to modulate ghrelin production and enhance vascularization to the cardia: proof of the concept in a porcine modelPubmed:25294792
- Embolization of Arterial Gastric Supply in Obesity (EMBARGO): an Endovascular Approach in the Management of Morbid Obesity. Proof of the Concept in the Porcine ModelPubmed:25511751
- Effect of Sleeve Gastrectomy Plus Side-to-Side Jejunoileal Anastomosis for Type 2 Diabetes Control in an Obese Rat ModelPubMed: 26202420
- Gastric Supply Manipulation to Modulate Ghrelin Production and Enhance Vascularization to the Cardia Proof of the Concept in a Porcine ModelPubMed: 25294792
- Elevation of Fasting Ghrelin in Healthy Human Subjects Consuming a High-Salt Diet: A Novel Mechanism of Obesity?Pubmed:27240398
- Effects of sleeve gastrectomy with jejuno-jejunal or jejuno-ileal loop onglycolipid metabolism in diabetic rats.pubmed:27621579
- Increased serum ghrelin in preeclampsia: is ghrelin a friend or a foe?pubmed:27321099
- The association between serum ghrelin levels and large-for-gestational-age fetuses in patients with gestational diabetes mellituspubmed:27791436
- Effect of lifestyle improvement program on the biomarkersof adiposity, inflammation and gut hormones in overweight/obese Asian Indians with prediabetespubmed:28620678
- Role of serotonin hormone in weight regain after sleeve gastrectomy pubmed:29228802
- EFFECT OF Garcinia cambogia EXTRACT ON SERUM LEPTIN, GHRELIN, ADIPONECTIN AND INSULIN LEVELS AND BODY WEIGHTS IN RATS FED WITH HIGH …Slovetres:Source
- The Relations Among Serum Ghrelin, Motilin and Gastric Emptying and Autonomic Function in Autoimmune GastritisPubmed:29753372
- The Role of Umbilical Cord Blood Concentration of IGF-I, IGF-II, Leptin, Adiponectin, Ghrelin, Resistin, and Visfatin in Fetal GrowthPubmed: 30282106
- The reparative effects of human adipose-derived mesenchymal stem cells in the chemotherapy damaged thymusPubmed: 30511904
- Grape Seed Proanthocyanidin Affects Lipid Metabolism via Changing Gut Microflora and Enhancing Propionate Production in Weaned PigsPubmed: 31175811
- Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise trainingPubmed: 31290696
- Ghrelin system is involved in improvements in glucose metabolism mediated by hyperbaric oxygen treatment in a streptozotocin‑induced type 1 diabetes mouse …Pubmed: 32901885
- Omega-3 offers better hypothalamus protection by decreasing POMC expression and elevating ghrelin hormone: a prospective trial to overcome methotrexate …Pubmed: 32424681
- Serum leptin level is associated with phase angle in CKD5 patients not undergoing dialysisPubmed: 30089154
- Physiological and Histopathological Effects of Electroporation Pulse on Stomach of Rats
- Effect of Nutritional Supplementation on Treatment of Anoestrus Swamp Buffalo Cows and Heifers
- Relationship Between Orexin-A and Insulin Resistance in Patients with Type 2 Diabetes Mellitus
- Altered Serum Marker of Adipokines Profile in Breast Cancer Women
- The Association of Pre-Pregnancy BMI On Leptin, Ghrelin, Adiponectin, and IGF-1 In Breast Milk, A Case Control Study34348809
- Nutritional Status, Selected Nutrients Intake and Their Relationship with the Concentration of Ghrelin and Adiponectin in Patients with Diabetic Nephropathy34959967
- Effect of Sleeve Gastrectomy, Roux-En-Y Gastric Bypass, and Ileal Transposition on Myocardial Ischaemia–Reperfusion Injury in Non-Obese Non-Diabetic Rats34903800
- The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammalPubmed:35379849
- Creating texturally tuneable, low calorie and palatable noodle-like food assemblies via microfluidics