Multiplex Assay Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. by CBA (Cytometric Bead Array) 
ATF1; ATF2; HFB1-GDNF; Astrocyte-Derived Trophic Factor; Glial Derived Neurotrophic Factor
Overview
Properties
- Product No.CMA043Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryCytokineApoptosisNeuro science
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 79-96 | 88 |
| EDTA plasma(n=5) | 81-90 | 86 |
| heparin plasma(n=5) | 91-103 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-96% | 93-105% | 78-104% | 78-102% |
| EDTA plasma(n=5) | 94-104% | 79-94% | 85-104% | 95-104% |
| heparin plasma(n=5) | 91-99% | 92-101% | 95-104% | 96-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.
Giveaways
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Citations
- Efficient Generation of Functionally Active Spinal Cord Neurons from Spermatogonial Stem Cellspubmed:27566610
- Safflower Extract and Aceglutamide Injection Promoting Recovery of Peripheral Innervations via Vascular Endothelial Growth Factor-B Signaling in Diabetic MicePMC5717862
- Acupuncture Alters Pro-infl ammatory Cytokines in the Plasma of Maternally Separated Rat Pupspubmed:28986807
- GDNF serum levels are found to be lower in opioid-maintained patientsISSN 2531-4122
- Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in RatsPubmed: 30974849
- The Effects of Nordic Walking With Poles With an Integrated Resistance Shock Absorber on Cognitive Abilities and Cardiopulmonary Efficiency in …Pubmed: 33192480
- Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡33389158
- The effect of biodegradable silk fibroin-based scaffolds containing glial cell line-derived neurotrophic factor (GDNF) on the corneal regeneration process34119551
- Silymarin constrains diacetyl-prompted oxidative stress and neuroinflammation in rats: involvements of Dyn/GDNF and MAPK signaling pathwayPubmed:35366745
- Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling …Pubmed:35585377