Multiplex Assay Kit for Glucose Transporter 4 (GLUT4) ,etc. by CBA (Cytometric Bead Array) 
SLC2A4; Solute carrier family 2, facilitated glucose transporter member 4; Glucose transporter type 4, insulin-responsive
Overview
Properties
- Product No.CMC023Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategorySignal transductionMetabolic pathwayEndocrinologyCardiovascular biology
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Glucose Transporter 4 (GLUT4) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Glucose Transporter 4 (GLUT4) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 78-93 | 81 |
| EDTA plasma(n=5) | 87-96 | 92 |
| heparin plasma(n=5) | 87-96 | 91 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucose Transporter 4 (GLUT4) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucose Transporter 4 (GLUT4) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glucose Transporter 4 (GLUT4) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-98% | 80-98% | 94-103% | 80-93% |
| EDTA plasma(n=5) | 86-101% | 88-95% | 89-101% | 89-98% |
| heparin plasma(n=5) | 85-93% | 79-90% | 88-95% | 89-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Glucose Transporter 4 (GLUT4) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Glucose Transporter 4 (GLUT4) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Glucose Transporter 4 (GLUT4) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Glucose Transporter 4 (GLUT4) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.
Giveaways
Increment services
Citations
- Glucose transporter-4 in white blood cells of young and old sled dogs: a model for human biomarker developmentCambridge: Source
- Conditioning causes an increase in glucose transporter-4 levels in mononuclear cells in sled dogsPubmed:25236492
- Maternal stress predicts altered biogenesis and the profile of mitochondrial proteins in the frontal cortex and hippocampus of adult offspring ratsPubMed: 26143539
- Antidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting DrugPubMed: 26262991
- Metabolic Features of Heart Failure with Different Etiology ofsp11075
- Hibiscus sabdariffa linn fruits methanolic extract and fractions mediated glucose uptake stimulation and glucose transporter 4 regulationDoi: 10.4103/jpnr.JPNR_14_18
- Effect of Noni Fruit Extract (Morinda citrifolia) on Glucose Intake to Diabetes Mellitus White Rat Muscle Tissue
- The Effects of Sambiloto Leaf Extract (Andrographis peniculata) on Blood Sugar Regulation: an In Vivo Study
- Effect of Bay Leaf Extract (Syzygium polyanthum) on Blood Sugar Regulation via GLUT4 Protein Regulation in White Rat Muscle Tissue Induced Aloxan
- Potential Combination of Tinospora crispa, Andrographis paniculata, Cinamomum burmanii, Syzygium polyanthum and Momordica charantia Extracts Against?¡