Multiplex Assay Kit for Glycated Hemoglobin A1c (HbA1c) ,etc. by CBA (Cytometric Bead Array) 
Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc
Overview
Properties
- Product No.CMA190Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Glycated Hemoglobin A1c (HbA1c) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Glycated Hemoglobin A1c (HbA1c) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 79-94 | 91 |
| EDTA plasma(n=5) | 88-96 | 91 |
| heparin plasma(n=5) | 81-93 | 87 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycated Hemoglobin A1c (HbA1c) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycated Hemoglobin A1c (HbA1c) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glycated Hemoglobin A1c (HbA1c) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-103% | 85-101% | 80-95% | 79-103% |
| EDTA plasma(n=5) | 84-103% | 96-103% | 80-97% | 83-99% |
| heparin plasma(n=5) | 91-99% | 95-104% | 80-92% | 84-98% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:HbA1c) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Glycated Hemoglobin A1c (HbA1c) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Glycated Hemoglobin A1c (HbA1c) ,etc. by CBA (Cytometric Bead Array) and unlabeled Glycated Hemoglobin A1c (HbA1c) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Glycated Hemoglobin A1c (HbA1c) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Glycated Hemoglobin A1c (HbA1c) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Glycated Hemoglobin A1c (HbA1c) ,etc. by CBA (Cytometric Bead Array) in the sample.
Giveaways
Increment services
Citations
- The Effect of Vitamin D Supplementation on Glycemic Control and Lipid Profile in Patients with Type 2 Diabetes MellitusPubMed: 26391639
- The Effect of Vitamin D Supplementation on Glycemic Control and Lipid Profile in Patients with Type 2 Diabetes Mellitus.pubmed:26391639
- Circulating Betatrophin and Hepatocyte Growth Factor in Type 2 Diabetic Patients: Their Relationship With Disease Prognosis405
- Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitropubmed:28105758
- d-Ribose as a Contributor to Glycated Haemoglobinpubmed:29033370
- Hypoglycemic effect of Hypericum attenuatum Choisy extracts on type 2 diabetes by regulating glucolipid metabolism and modulating gut microbiota
- Plasma Cellular Hypoxia, Mitochondrial Dysfunction, Disease Risk and Prognostic Factors in Type 2 Diabetic Patients in Saudi Arabia.
- Stressors, allostatic load, and health outcomes among women hotel housekeepers: A pilot studyPubmed: 30615593
- Parabacteroides distasonis Alleviates Obesity and Metabolic Dysfunctions via Production of Succinate and Secondary Bile AcidsPubmed: 30605678
- Gymnemic acid alleviates type 2 diabetes mellitus and suppresses endoplasmic reticulum stress in vivo and in vitro
- 1H Nuclear Magnetic Resonance (NMR)-Based Cerebrospinal Fluid and Plasma Metabolomic Analysis in Type 2 Diabetic Patients and Risk Prediction for Diabetic …Pubmed: 31248127
- Gymnemic Acid Alleviates Inflammation and Insulin Resistance via PPARδ-and NFκB-mediated pathways in db/db mice.
- Duodenal-Jejunal Bypass Ameliorates Type 2 Diabetes Mellitus by Activating Insulin Signaling and Improving Glucose Utilization in the BrainPubmed: 31605365
- Modulation of gut microbiota contributes to effects of intensive insulin therapy on intestinal morphological alteration in high-fat-diet-treated micePubmed: 31749050
- Inhibition of Receptor for Advanced Glycation End Products as New Promising Strategy Treatment in Diabetic RetinopathyPubmed: 32165929
- Activation of a Specific Gut Bacteroides-Folate-Liver Axis Benefits for the Alleviation of Nonalcoholic Hepatic SteatosisPubmed: 32783933
- The effects of different intensity endurance and resistance exercise on diabetic-related blood profiles in impaired glucose tolerance mice
- Relation of pharmacopenile duplex ultrasonography parameters and glycated hemoglobin (HbA1c) in diabetic patients with erectile dysfunction33334711
- Identification of Key Contributive Compounds in a Herbal Medicine: A Novel Mathematic¡ªBiological Evaluation Approach
- The Roles of Liver Inflammation and the Insulin Signaling Pathway in PM2. 5 Instillation-Induced Insulin Resistance in Wistar Rats34745386
- Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced MicePubmed:35740008
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