Multiplex Assay Kit for Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) ,etc. by CBA (Cytometric Bead Array) Homo sapiens (Human) Protein, Antibody, Assaykit, Service

G3PD; GAPD; Peptidyl-cysteine S-nitrosylase GAPDH

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 559.00 US$ 798.00
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  • Multiplex Assay Kit for Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-103 101
EDTA plasma(n=5) 78-102 92
heparin plasma(n=5) 78-89 86

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-99% 93-102% 80-97% 93-101%
EDTA plasma(n=5) 99-105% 80-95% 94-101% 81-94%
heparin plasma(n=5) 98-105% 90-101% 91-99% 86-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

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  • Adhesion of Toxoplasma gondii tachyzoite-infected vehicle leukocytes to capillary endothelial cells triggers timely parasite egressionpubmed:28720868
  • Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipidsPubmed: 30237167
  • Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogelsPubmed: 30343256
  • Influence of Magnetite Nanoparticles and Quantum Dots on the Expression of Reference Genes in Peripheral Blood CellsPubmed: 30488202
  • Discovery, Optimization and Target Identification of Novel Potent Broad-Spectrum Antiviral InhibitorsPubmed: 30938999
  • Methionine enkephalin (MENK) regulates the immune pathogenesis of type 2 diabetes mellitus via the IL-33/ST2 pathwayPubmed: 31078923
  • Rationale for a Combination Therapy Consisting of MCL1-and MEK-Inhibitors in Acute Myeloid LeukemiaPubmed: 31718075
  • Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor …Pubmed: 32046779
  • Silence of FAM83H-AS1 promotes chemosensitivity of gastric cancer through Wnt/β-catenin signaling pathwayPubmed: 32028241
  • Ginger and turmeric lipid-solubles attenuate heated oil-induced oxidative stress in the brain via the upregulation of NRF2 and improve cognitive function in ratsPubmed: 33170419
  • Evaluation of the effect of herbal extracts and their bioactive compounds against motion sickness by regulating neurotransmitter levels in vitro and in vivo
  • Regulation of hepatic lipid metabolism in CB1-/-and Abcb4-/--HBV surface protein transgenic mice
  • BMI1-Inhibitor PTC596 in Combination with MCL1 Inhibitor S63845 or MEK Inhibitor Trametinib in the Treatment of Acute Leukemia. Cancers 2021, 13, 58133540760
  • Omega-3 Eicosapentaenoic Acid Reduces Prostate Tumor Vascularity33262291
  • Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells34837026
  • Curcumin alters distinct molecular pathways in breast cancer subtypes revealed by integrated miRNA/mRNA expression analysisPubmed:34981672
  • Retinoic acid receptor beta protects striatopallidal medium spiny neurons from mitochondrial dysfunction and neurodegenerationPubmed:35151792
  • Arabidopsis GELP7 functions as a plasma membrane-localized acetyl xylan esterase, and its overexpression improves saccharification efficiencyPubmed:35577991

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