Multiplex Assay Kit for Gonadotropin Releasing Hormone (GnRH) ,etc. by CBA (Cytometric Bead Array) 
GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1
Overview
Properties
- Product No.CMA843Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryEndocrinologyReproductive scienceNeuro scienceGenetic scienceHormone metabolism
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Gonadotropin Releasing Hormone (GnRH) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Gonadotropin Releasing Hormone (GnRH) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 84-93 | 90 |
| EDTA plasma(n=5) | 90-104 | 101 |
| heparin plasma(n=5) | 94-105 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gonadotropin Releasing Hormone (GnRH) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gonadotropin Releasing Hormone (GnRH) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gonadotropin Releasing Hormone (GnRH) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-101% | 79-88% | 91-105% | 82-90% |
| EDTA plasma(n=5) | 92-101% | 97-105% | 85-93% | 84-98% |
| heparin plasma(n=5) | 80-89% | 80-90% | 82-94% | 93-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:GnRH) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Gonadotropin Releasing Hormone (GnRH) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Gonadotropin Releasing Hormone (GnRH) ,etc. by CBA (Cytometric Bead Array) and unlabeled Gonadotropin Releasing Hormone (GnRH) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Gonadotropin Releasing Hormone (GnRH) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Gonadotropin Releasing Hormone (GnRH) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Gonadotropin Releasing Hormone (GnRH) ,etc. by CBA (Cytometric Bead Array) in the sample.
Giveaways
Increment services
Citations
- Maternal exposure to dioxin imprints sexual immaturity of the pups through fixing the status of the reduced expression of hypothalamic gonadotropin-releasing …Pubmed:24132183
- Heavy Resistance Training and Supplementation With the Alleged Testosterone Booster Nmda has No Effect on Body Composition, Muscle Performance, and Serum Hormones Associated With the Hypothalamo-Pituitary-Gonadal Axis in Resistance-Trained MalesPubmed:24570624
- Perfluorooctane sulfonate effects on the reproductive axis in adult male ratsPubmed:25171141
- Possible role of serotonin and neuropeptide Y on the disruption of the reproductive axis activity by perfluorooctane sulfonatePubmed:25623392
- Chronic exposure of female mice to an environmental level of perfluorooctane sulfonate suppresses estrogen synthesis through reduced histone H3K14 acetylation of the StAR promoter leading to deficits in follicular development and ovulationPubMed: 26358002
- Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in MicePubmed:26771609
- OP0019-HPR Effectiveness of A Progressive Resistance Strength Program on Hand Osteoarthritis: A Randomised Controlled Trial60.1.abstract
- OP0021-HPR Sleep Disorders in Patients with Rheumatoid Arthritis and Relationship with Disease Activity60.3.abstract
- Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspringpubmed:27803384
- OP0020 The Association of Gonadotropin-Releasing Hormone and Cytokines in Rheumatoid Arthritiscontent:75
- Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axispubmed:28731686
- Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons10.1093:toxsci
- Nectin-like molecule 2, a necessary sexual maturation regulator, participates in congenital hypogonadotropic hypogonadismPubmed: 32535046
- Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat StressPubmed: 32685488
- Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis33422655
- Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice34730391