Multiplex Assay Kit for Growth Hormone (GH) ,etc. by CBA (Cytometric Bead Array) Canis familiaris; Canine (Dog) Protein, Antibody, Assaykit, Service

GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 605.00 US$ 864.00
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  • Multiplex Assay Kit for Growth Hormone (GH) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Growth Hormone (GH) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Growth Hormone (GH) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Growth Hormone (GH) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-104 94
EDTA plasma(n=5) 99-105 102
heparin plasma(n=5) 94-101 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone (GH) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone (GH) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Growth Hormone (GH) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-103% 95-103% 95-105% 85-104%
EDTA plasma(n=5) 98-105% 87-101% 85-93% 85-101%
heparin plasma(n=5) 84-101% 81-98% 92-101% 78-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Growth Hormone (GH) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Growth Hormone (GH) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Growth Hormone (GH) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Growth Hormone (GH) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Growth Hormone (GH) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • The effect of peripheral administration of growth hormone on AD-like cognitive deficiency in NBM-lesioned ratsPubMed: 19765635
  • Changes in the Somatotrophic Axis in Genetically Identical DogsPubMed: 21740269
  • The efficacy of IGF-I receptor monoclonal antibody against human gastrointestinal carcinomas is independent of k-ras mutation statusPubMed: 21642381
  • Growth hormone expression and neuroprotective activity in a quail neural retina cell lineScienceDirect: S0016648009002457
  • Periodic 48 h feed withdrawal improves glucose tolerance in growing pigs by enhancing adipogenesis and lipogenesisNutrition&Metabolism: source
  • Treatment of cervical metaphyseal fractures due to delayed ossification in an adult dog with hypopituitarismBmj: Source
  • GH Deficiency in a Dalmatian Puppy with MegaesophagusPubmed: 24051259
  • Functional expression of bovine growth hormone gene in Pleurotus eryngiiSpringer: Source
  • Profil Growth Hormone Sapi Bali Betina Pada Tiga Tipe Lahan Pemeliharaan Di Provinsi BaliUnud:Source
  • Effect of prill fat supplementation on hormones, milk production and energy metabolites during mid lactation in crossbred cowsVeterinaryworld:Source
  • Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetesPubMed: 26379831
  • Melatonin modulates monochromatic light-induced GHRH expression in the hypothalamus and GH secretion in chicksPubmed:26948666
  • Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens journalprintahead:87
  • Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickensview:87
  • Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calvespublication:306322503
  • TRANSMISI GEN PhGH DAN PERFORMA PERTUMBUHAN IKAN LELE AFRIKA (Clarias gariepinus) TRANSGENIK GENERASI KETIGA1329
  • Assessment of feeding varying levels of Metabolizable energy and protein on performance of transition Murrah buffaloespubmed:28785907
  • Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Dietarticle:10.1007
  • Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken10.159:1806-9061-2016-0285
  • 单色绿光对鸡胚褪黑激素合成的影响DOI: 10.11843/j.issn.0366-6964.2017.05.019
  • Effects of Bariatric Surgery on Change of Brown Adipocyte Tissue and Energy Metabolism in Obese Micepubmed:28853013
  • Kadar Hormon Pertumbuhan Sapi Bali Lebih Rendah di Nusa Penida Daripada Daerah Bali Lainnya(LEVELS OF GROWTH HORMONE BALI CATTLE IN NUSA PENIDA LOWER THAN OTHER BALI REGIONS)10.19087/jveteriner.2017.18.2.226
  • Factors influencing growth hormone levels of Bali cattle in Bali, Nusa Penida, and Sumbawa Islands, Indonesia.pubmed:29184372
  • Effects of Dietary Lysine Levels on the Plasma Concentrations of Growth‐Related Hormones in Late‐Stage Finishing Pigs10.5772/intechopen.68545
  • Decreased Hepatic Lactotransferrin Induces Hepatic Steatosis in Chronic Non-Alcoholic Fatty Liver Disease ModelPubmed:29975946
  • Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickensPubmed:29373588
  • Effects of Intrauterine Growth Retardation on Growth, Meat Quality and Muscle Fiber Composition of Pigs.:
  • Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in MicePubmed: 30404008
  • Physiological Performance of Crossbred Cattle Calves (Karan Fries) under Different Housing Conditions during Different Seasons
  • Melatonin Receptor Mel1b‐and Mel1c‐mediated Green Light Induced the Secretion of Growth Hormone in Anterior Pituitary of ChicksPubmed: 31111492
  • Dietary lysine affects amino acid metabolism and growth performance, which may not involve the GH/IGF-1 axis, in young growing pigsPubmed: 31922564
  • The evaluation of leukocyte-platelet rich fibrin as an anti-inflammatory autologous biological additivePubmed: 31287125
  • RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose HomeostasisPubmed: 32328034
  • Isolated growth hormone deficiency in a Chihuahua with a GH1 mutationPubmed: 32646299
  • Controlled elevated temperatures during early-mid gestation cause placental insufficiency and implications for fetal growth in pregnant pigs33244103
  • Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves33610512
  • GHR is involved in gastric cell growth and apoptosis via PI3K/AKT signalling33492754
  • Effects of Concentrate and Bypass Fat Supplementations on Growth Performance, Blood Profile, and Rearing Cost of Feedlot Buffaloes34359233

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