Multiplex Assay Kit for Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ,etc. by CBA (Cytometric Bead Array) 
HB-EGF; DTR; DTS; DTSF; HEGFL; Diphtheria Toxin Receptor; Proheparin-binding EGF-like growth factor; Diphtheria toxin receptor
Overview
Properties
- Product No.CMB479Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryCytokineTumor immunityInfection immunity
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 91-101 | 97 |
| EDTA plasma(n=5) | 83-93 | 87 |
| heparin plasma(n=5) | 79-96 | 83 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-101% | 95-102% | 87-95% | 92-104% |
| EDTA plasma(n=5) | 87-94% | 90-102% | 84-92% | 97-105% |
| heparin plasma(n=5) | 88-95% | 94-105% | 78-99% | 84-95% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.
Giveaways
Increment services
Citations
- Astrocyte ERK phosphorylation precedes K+-induced swelling but follows hypotonicity-induced swelling.PubMed: 21118399
- Cypermethrin Induces Astrocyte Apoptosis by the Disruption of the Autocrine/Paracrine Mode of Epidermal Growth Factor Receptor SignalingPubmed: source
- 軽度炎症性膀胱痛マウスモデルの構築とNGFの関与Jstage:Source
- A novel method for assessing bladder-related pain reveals the involvement of nerve growth factor in painassociated with cyclophosphamide-induced chronic cystitis in mice.Pubmed:25820250
- 2 型糖尿病モデルラットにおける糖尿病性腎症の進行とHB-EGF の関連性17844
- Heparin-binding epidermal growth factor contributes to COPD disease severity by modulating airway fibrosis and pulmonary epithelial–mesenchymal transitionPubmed:29581578
- CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17
- Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growthPubmed: 30769285
- Polarization of ADAM17‐driven EGFR signalling in electric field‐guided collective migration of epidermal sheetsPubmed: 33164313
- 象牙芽細胞の枯渇は象牙芽細胞分化と象牙質形成を誘導する