Multiplex Assay Kit for Hepatocyte Growth Factor (HGF) ,etc. by CBA (Cytometric Bead Array) 
HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen
Overview
Properties
- Product No.CMA047Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryCytokineTumor immunityHepatology
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Hepatocyte Growth Factor (HGF) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Hepatocyte Growth Factor (HGF) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 84-92 | 87 |
| EDTA plasma(n=5) | 81-101 | 93 |
| heparin plasma(n=5) | 99-105 | 102 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Growth Factor (HGF) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Growth Factor (HGF) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hepatocyte Growth Factor (HGF) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-94% | 82-99% | 93-101% | 89-104% |
| EDTA plasma(n=5) | 85-92% | 97-104% | 98-105% | 91-98% |
| heparin plasma(n=5) | 90-97% | 79-103% | 80-93% | 80-89% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Hepatocyte Growth Factor (HGF) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Hepatocyte Growth Factor (HGF) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Hepatocyte Growth Factor (HGF) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Hepatocyte Growth Factor (HGF) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.
Giveaways
Increment services
Citations
- Sphingosine kinase-1 protects transplanted mesenchymal stem cells and improves the performance of the infarcted heartLWW: 01000
- Low temperature of radiofrequency ablation at the target sites can facilitate rapid progression of residual hepatic VX2 carcinomaBioMed: 14795876
- Gene Transfer of c-met Confers Protection Against d-Galactosamine/Lipopolysaccharide-Induced Acute Liver FailureSpringerLink: k22823l138x2344x
- Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic ChildrenPubMed: PMC3694825
- Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD)PubMed: PMC3762604
- BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopausePubMed: PMC3640998
- Effect of Mesenchymal Stem Cells on Doxorubicin-Induced FibrosisPubMed: PMC3584430
- Proinflammatory Cytokines (IL-1α, IL-6) and Hepatocyte Growth Factor in Patients with Alcoholic Liver CirrhosisPubMed: 26448742
- Hypoxia-induced secretion of IL-11 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphomaPubMed: 26695151
- Cell type–dependent variation in paracrine potency determines therapeutic efficacy against neonatal hyperoxic lung injuryPubMed: 25863963
- Silymarin-loaded Eudragit® RS100 nanoparticles improved the ability of silymarin to resolve hepatic fibrosis in bile duct ligated ratsPubmed:27261582
- Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosisPubmed:27186801
- Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cellsPubmed:27323064
- Changes in HGF levels after a progressive test before and after Tabata HIIT programprofile:Pawel_Wolanski3
- Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats10.1186/s13287-017-0739-3
- Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c‑Met/PI3K/AKT in vitro10.3892/ol.2017.7301
- Overexpression of c-Met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure.pubmed:28679425
- Pulmonary pericytes regulate lung morphogenesisPubmed:29934496
- Interleukin-1 receptor antagonist-mediated neuroprotection by umbilical cord-derived mesenchymal stromal cells following transplantation into a rodent stroke …Pubmed:29650950
- Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitroPubmed:29375714
- Dynamics of Expression of Cytokine Genes and Macrophage Content in the Lungs and Kidneys after Subtotal Hepatectomy in RatsPubmed:29796817
- Inherent control of hepatocyte proliferationInherent control of hepatocyte proliferation after subtotal liver resectionPubmed: 31297922
- What is the Best Degree of Hyaluronic Acid Crosslinking in Increasing Growth Factors Level of Platelet-Rich Fibrin Lysate?Pubmed: 31239605
- Efficacy of autologous bone marrow mononuclear cell transplantation in dogs with chronic spinal cord injuryPubmed: 32821665
- Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care33469783
- Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling …Pubmed:35585377