Multiplex Assay Kit for Hydroxyproline (Hyp) ,etc. by CBA (Cytometric Bead Array) Pan-species (General) Protein, Antibody, Assaykit, Service

(2S,4R)-4-Hydroxyproline; L-Hydroxyproline(C5H9O3N)

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 669.00 US$ 955.00
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  • Multiplex Assay Kit for Hydroxyproline (Hyp) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Hydroxyproline (Hyp) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of Hydroxyproline (Hyp) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Hydroxyproline (Hyp) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-90 85
EDTA plasma(n=5) 80-91 86
heparin plasma(n=5) 78-99 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hydroxyproline (Hyp) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hydroxyproline (Hyp) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hydroxyproline (Hyp) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-96% 98-105% 85-99% 87-99%
EDTA plasma(n=5) 80-102% 94-102% 80-92% 97-105%
heparin plasma(n=5) 88-95% 89-103% 97-105% 92-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Hydroxyproline (Hyp) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Hydroxyproline (Hyp) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Hydroxyproline (Hyp) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Hydroxyproline (Hyp) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Hydroxyproline (Hyp) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Increment services

Citations

  • Spleen-Derived Interleukin-10 Downregulates the Severity of High-Fat Diet-Induced Non-Alcoholic Fatty Pancreas DiseasePubMed: PMC3532347
  • Prediction of fibrosis in hepatitis C patients: assessment using hydroxyproline and oxidative stress biomarkersSpringer: Source
  • Royal jelly affects collagen crosslinking in bone of ovariectomized ratsSciencedirect:S1756464614000346
  • Tanshinone IIA ameliorates bleomycin-induced pulmonary fibrosis and inhibits transforming growth factor-beta-β–dependent epithelial to mesenchymal transitionPubMed: 25911951
  • Theophylline, an old drug with multi-faceted effects: Its potential benefits in immunological liver injury in ratsPubMed: 26165752
  • Effects of 4% Icodextrin on Experimental Spinal Epidural Fibrosispdf:JTNEPUB_15079_online.pdf
  • Celecoxib attenuates depressive-like behavior associated with immunological liver injury inC57BL/6 mice through TNF-α and NF-κb dependent mechanisms.pubmed:27580523
  • Sustained-released mixture of vascular endothelial growth factor 165 and fibrin glue strengthens healing of ileal anastomoses in a rabbit model with …doi.org:10.4174
  • Effects of panax notoginseng saponin on the pathological ultrastructure and serum IL‐6 and IL‐8 in pulmonary fibrosis in rabbitsPubmed:29932250
  • Renin–angiotensin system inhibition ameliorates CCl-induced liver fibrosis in mice through the inactivation of nuclear transcription factor kappa B10.1139:cjpp-2017-0728
  • Pirfenidone suppresses bleomycin‑induced pulmonary fibrosis and periostin expression in rats10.3892:etm.2018.6378
  • Therapeutic effect of hepatocyte growth factor-overexpressing bone marrow-derived mesenchymal stem cells on CCl 4-induced hepatocirrhosis.
  • The effects of platelet‐rich‐plasma gel application to the colonic anastomosis in hyperthermic intraperitoneal chemotherapy: An experimental rat modelPubmed: 31448554
  • Pantoea agglomerans chronic exposure induces epithelial-mesenchymal transition in human lung epithelial cells and mice lungsPubmed: 32146192
  • Is platelet-rich plasma improves the anastomotic healing in hyperthermic intraperitoneal chemotherapy with oxaliplatin: an experimental rat studyPubmed: 32051817
  • Protective Effects of Contractubex® on Stricture Formation after Experimental Corrosive Esophageal Burns in RatsPubmed: 32654881
  • Effect of Oral Channa striatus in Laparotomy Wound Healing of Malnourished Rats.
  • Handgrip Strength and Vitamin D as Predictors of Liver Fibrosis and Malnutrition in Chronic Hepatitis C Patients33884041
  • Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies33999928
  • Curdione and Schisandrin C Synergistically Reverse Hepatic Fibrosis via Modulating the TGF-β Pathway and Inhibiting Oxidative Stress34858986

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