Multiplex Assay Kit for Immunoglobulin E (IgE) ,etc. by CBA (Cytometric Bead Array) 
IGHE; Immunoglobulin Heavy Constant Epsilon; Ig epsilon chain C region
Overview
Properties
- Product No.CMA545Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryInfection immunityImmune moleculeHematologyAutoimmunity
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Immunoglobulin E (IgE) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Immunoglobulin E (IgE) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 88-98 | 94 |
| EDTA plasma(n=5) | 87-96 | 92 |
| heparin plasma(n=5) | 78-103 | 99 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin E (IgE) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin E (IgE) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin E (IgE) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-105% | 79-92% | 81-98% | 78-97% |
| EDTA plasma(n=5) | 92-101% | 85-92% | 94-102% | 96-103% |
| heparin plasma(n=5) | 84-99% | 89-97% | 82-99% | 85-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Immunoglobulin E (IgE) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Immunoglobulin E (IgE) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Immunoglobulin E (IgE) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Immunoglobulin E (IgE) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.
Giveaways
Increment services
Citations
- IL17A gene polymorphisms, serum IL-17A and IgE levels, and hepatocellular carcinoma risk in patients with chronic hepatitis B virus infectionPubMed: 23280722
- IL21 and IL21R polymorphisms and their interactive effects on serum IL-21 and IgE levels in patients with chronic hepatitis B virus infectionPubMed: 23354321
- Passive transfer of tumour-derived MDSCs inhibits asthma-related airway inflammationPubmed: 24313384
- Topical Lyogel Containing Corticosteroid Decreases IgE Expression and Enhances the Therapeutic Efficacy Against Atopic EczemaPubMed: 25511806
- Reduced airway microbiota diversity is associated with elevated allergic respiratory inflammationPubMed: 26123423
- The protective role of vitamin D3 in a murine model of asthma via the suppression of TGF-β/Smad signaling and activation of the Nrf2/HO-1 pathway.pubmed:27484042
- Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in micepubmed:28250847
- Catalpol alleviates ovalbumin-induced asthma in mice: Reduced eosinophil infiltration in the lungpubmed:27992791
- Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in micepubmed:28122397
- Protective effects of astragaloside IV against ovalbumin-induced allergic rhinitis are mediated by T-box protein expressed in T cells/GATA-3 and forkhead box protein 3/retinoic acid-related orphan nuclear receptor γt.pubmed:28586019
- Commensal bacteria aggravate allergic asthma via NLRP3/IL-1β signaling in post-weaning mice10.1016:j.jaut.2018.07.003
- Sub-chronic inhalation of reclaimed water-induced fibrotic lesion in a mouse modelPubmed:29655095
- Investigation of hypersensitivity potential of diacetyl by determining cytokine profilesPubmed:29233007
- Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary diseasePubmed:29950127
- Effects of catalpol on bronchial asthma and its relationship with cytokinesPubmed: 30536454
- Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat ModelPubmed: 30781605
- Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats
- Antiallergical Effect of New Combined Nazal Aerodisperse System in the Conditions of Experimental Allergic Rhinitis
- Inhibitory effects of catalpol coordinated with budesonide and their relationship with cytokines and Interleukin-13 expressionPubmed: 31737193
- Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injectionPubmed: 32302806
- Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in ratPubmed: 32395767
- Synergistic Phage-surfactant Combination Clears IgE-promoted Staphylococcus aureus Aggregation in Vitro and Enhances the Effect in VivoPubmed: 32335278
- Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota
- Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction
- Biomarkers of Allergic Asthma and their Association with Serum Parameters
- Qingfei oral liquid inhibited autophagy to alleviate inflammation via mTOR signaling pathway in RSV-infected asthmatic mice33706133
- Trace endotoxin in reclaimed water is only one of the risk sources in subchronic inhalation exposure34090073
- Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity
- Roles Played by the PI3K/Akt/HIF-1α Pathway and IL-17A in the Chinese Subtype of Chronic Sinusitis with Nasal PolypsPubmed:35075349
- Dapagliflozin mitigates ovalbumin-prompted airway inflammatory-oxidative successions and associated bronchospasm in a rat model of allergic asthmaPubmed:35549595