Multiplex Assay Kit for Immunoglobulin M (IgM) ,etc. by CBA (Cytometric Bead Array) Chicken (Gallus) Protein, Antibody, Assaykit, Service

IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chain C region

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 447.00 US$ 638.00
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  • Multiplex Assay Kit for Immunoglobulin M (IgM) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Immunoglobulin M (IgM) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Immunoglobulin M (IgM) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Immunoglobulin M (IgM) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-94 89
EDTA plasma(n=5) 83-104 94
heparin plasma(n=5) 97-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin M (IgM) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin M (IgM) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin M (IgM) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-103% 98-105% 88-101% 94-101%
EDTA plasma(n=5) 79-91% 84-98% 87-96% 94-101%
heparin plasma(n=5) 80-94% 79-97% 94-105% 83-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Immunoglobulin M (IgM) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Immunoglobulin M (IgM) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Immunoglobulin M (IgM) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Immunoglobulin M (IgM) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Immunoglobulin M (IgM) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88Pubmed: 24570422
  • Speci?c Humoral Immune Response Induced by Propionibacterium acnes Can Prevent Actinobacillus pleuropneumoniae Infection in MicePubmed:24429068
  • INFLUENCE OF ZINCOVIT DROP (NUTRITIONAL FOOD SUPPLEMENT) ON IMMUNE SYSTEM IN NORMAL AND CYCLOPHOSPHAMIDE INTOXICATED WISTAR RATSjournals:3790
  • Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers10.108:09540105.2017.1311308
  • Detection of streptococcus pyogenes antibodies in acute idiopathic urticariaISSN:1940-5901/IJCEM0053690
  • Effects of and on growth performance, immune function and volatile fatty acid level of caecal digesta in broilers10.1080:09540105.2018.1457013
  • Altered immunoglobulins (A and G) in Ghanaian patients with type 2 diabetesPubmed:29623201
  • Effects of polysaccharides from Yingshan Yunwu tea on meat quality, immune status and intestinal microflora in chickensPubmed: 32224178
  • Physicochemical characterization and immunomodulatory activity of sulfated Chinese yam polysaccharidePubmed: 33010421
  • Association Between Serum Selenium Level and Subclinical Mastitis in Dairy CattlePubmed: 32583225
  • IMMUNOSTIMULATORY EFFECT OF MORINGA OLEIFERA LEAVES EXTRACT ON CYCLOPHOSPHAMIDE-INDUCED IMMUNOSUPPRESSION IN MICE
  • Comparison of immunomodulatory effects of three polysaccharide fractions from Lentinula edodes water extracts
  • Effects of chitooligosaccharide supplementation on laying performance, egg quality, blood biochemistry, antioxidant capacity and immunity of laying hens during the …
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  • Celecoxib ameliorates liver cirrhosis via reducing inflammation and oxidative stress along spleen-liver axis in rats33577848
  • Baicalin relieves Mycoplasma pneumoniae infection?induced lung injury through regulating microRNA?221 to inhibit the TLR4/NF?¦ÊB signaling pathway34109422
  • Trace endotoxin in reclaimed water is only one of the risk sources in subchronic inhalation exposure34090073
  • The suppression of Brd4 inhibits peripheral plasma cell differentiation and exhibits therapeutic potential for systemic lupus erythematosus
  • Immunostimulatory Effect of Moringa oleifera Extracts on Cyclophosphamide-induced Immunosuppressed Mice
  • Dietary Supplementation of Inulin Ameliorates Subclinical Mastitis via Regulation of Rumen Microbial Community and Metabolites in Dairy Cows34494854
  • Sulfated modification enhances the immunomodulatory effect of Cyclocarya paliurus polysaccharide on cyclophosphamide-induced immunosuppressed mice through …34865774
  • Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein ComponentsPubmed:35438093

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