Multiplex Assay Kit for Laminin (LN) ,etc. by CBA (Cytometric Bead Array) 
LAM
- UOM
- FOB US$ 472.00 US$ 490.00 US$ 517.00 US$ 553.00 US$ 590.00 US$ 644.00 US$ 726.00 US$ 907.00
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Overview
Properties
- Product No.CMA082Po
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Laminin (LN) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Laminin (LN) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 97-105 | 102 |
| EDTA plasma(n=5) | 88-102 | 96 |
| heparin plasma(n=5) | 79-88 | 85 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Laminin (LN) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Laminin (LN) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Laminin (LN) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-95% | 94-105% | 83-91% | 78-99% |
| EDTA plasma(n=5) | 83-95% | 92-105% | 97-104% | 80-92% |
| heparin plasma(n=5) | 83-101% | 83-101% | 83-92% | 88-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Laminin (LN) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Laminin (LN) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Laminin (LN) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Laminin (LN) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.
Giveaways
Increment services
Citations
- Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jellyPubmed: 24739658
- Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented mediaPubmed:Pmc4130592
- Augmented expression of urokinase plasminogen activator and extracellular matrix proteins associates with multiple myeloma progressionPubmed:24807734
- Investigation?of?diagnostic?potentials?of?nine?different?biomarkers?in?endometriosis.Pubmed:24813083
- Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile HemangiomasPubMed: 26430624
- Modified Citrus Pectin stops progression of liver fibrosis by inhibiting galactin-3 and inducing apoptosis of stellate cellsDoi: Abs
- Clinical significance of serum laminin and typeâIV collagen levels in cutaneous melanoma patientsPubmed:27330797
- Key Matrix Proteins Within the Pancreatic Islet Basement Membrane Are Differentially Digested During Human Islet Isolation.pubmed:27456745
- Modulatory Effects of Chemoradiation on Angiogenic Factors and Laminin in Cervical Cancer: Link with Treatment Responsepubmed:29172262
- Coordination Among Lipid Droplets, Peroxisomes and Mitochondria Regulates Energy Expenditure Through the CIDE-ATGL-PPARα Pathway in AdipocytesPubmed:29986925
- Evaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis10.1007:s00217-018-3119-z
- High amplitude stretching of ATII cells and fibroblasts results in profibrotic effectsPubmed: 31290711
- Fabrication and Evaluation of a Xenogeneic Decellularized Nerve-Derived Material: Preclinical Studies of a New Strategy for Nerve RepairPubmed: 31758411
- Untersuchungen und semiquantitative Symmetrie-Analysen zur Extrazellulärmatrix von Kalbs-Stimmlippen
- CLINICAL SIGNIFICANCE OF LAMININ AND ELASTINE LEVELS IN CHILDREN WITH UNDIFFERENTIATED CONNECTIVE TISSUE DISEASE