Multiplex Assay Kit for Lipopolysaccharide Binding Protein (LBP) ,etc. by CBA (Cytometric Bead Array) Homo sapiens (Human) Protein, Antibody, Assaykit, Service

LPS-Binding Protein

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 559.00 US$ 798.00
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  • Multiplex Assay Kit for Lipopolysaccharide Binding Protein (LBP) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Lipopolysaccharide Binding Protein (LBP) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Lipopolysaccharide Binding Protein (LBP) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Lipopolysaccharide Binding Protein (LBP) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-105 95
EDTA plasma(n=5) 87-103 99
heparin plasma(n=5) 78-102 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipopolysaccharide Binding Protein (LBP) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipopolysaccharide Binding Protein (LBP) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lipopolysaccharide Binding Protein (LBP) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-105% 90-98% 84-97% 80-90%
EDTA plasma(n=5) 93-105% 92-102% 89-104% 78-101%
heparin plasma(n=5) 87-94% 80-94% 99-105% 83-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Lipopolysaccharide Binding Protein (LBP) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Lipopolysaccharide Binding Protein (LBP) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Lipopolysaccharide Binding Protein (LBP) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Lipopolysaccharide Binding Protein (LBP) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Lipopolysaccharide Binding Protein (LBP) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • A Marker of Endotoxemia Is Associated With Obesity and Related Metabolic Disorders in Apparently Healthy ChineseDiabetes: source
  • Oral serum-derived bovine immunoglobulin improves duodenal immune reconstitution and absorption function in patients with HIV enteropathyPubMed: PMC3754419
  • Gut microbiome composition is linked to whole grain-induced immunological improvementsPubmed: 23038174
  • High ambient temperature alleviates the inflammatory response and growth depression in pigs challenged with Escherichia coli lipopolysaccharidePubmed: 24792207
  • The effect of normovolemic modified ultrafiltration on inflammatory mediators, endotoxins, terminal complement complexes and clinical outcome in high-risk cardiac surgery patientsPubmed: 23429100
  • A gut microbiota-targeted dietary intervention for amelioration of chronic inflammation underlying metabolic syndrome.Pubmed: 24117923
  • Frequency and Severity of Cirrhotic Cardiomyopathy and Its Possible Relationship with Bacterial EndotoxemiaPubmed: 23907333
  • Circulating pathogen associated molecular pattern – Binding proteins and High Mobility Group Box protein 1 in nascent metabolic syndrome: Implications for cellular Toll-like receptor activityPubmed:25063948
  • Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical studyPubmed:25652121
  • Increased adipose tissue secretion of Fetuin-A, lipopolysaccharide-binding protein and high-mobility group box protein 1 in metabolic syndromePubMed: 25978344
  • Systemic levels of human β-defensin 1 are elevated in patients with cirrhosisPubMed: 26751578
  • Comparative proteomics of milk fat globule membrane in goat colostrum and mature milkPubmed:27173528
  • High serum lipopolysaccharide binding protein is associated with increased mortality in patients with decompensated cirrhosispubmed:27712029
  • Modulation of gut microbiota and gut-generated metabolites by bitter melon results in improvement in the metabolic status in high fat diet-induced obese rats10.1016/j.jff.2017.12.050
  • Adamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains.pubmed:28512356
  • A standardized extract of the fruit of Hovenia dulcis alleviated alcohol-induced hangover in healthy subjects with heterozygous ALDH2: A randomized, controlled, crossover trial.pubmed:28750942
  • On a Western diet, APOE ɛ4 is associated with low innate immune sensing, but not APOE ɛ3Pubmed:29928926
  • Influence of yeast culture and feed antibiotics on ruminal fermentation and site and extent of digestion in beef heifers fed high grain rationsPubmed:30060086
  • Using ruminally protected and nonprotected active dried yeast as alternatives to antibiotics in finishing beef steers: growth performance, carcass traits, blood …Pubmed: 30184125
  • Evaluation of the effect of Lactobacillus reuteri V3401 on biomarkers of inflammation, cardiovascular risk and liver steatosis in obese adults with metabolic …Pubmed: 30453950
  • Oridonin protects LPS-induced acute lung injury by modulating Nrf2-mediated oxidative stress and Nrf2-independent NLRP3 and NF-κB pathways
  • High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights …
  • Ruminally protected and unprotected Saccharomyces cerevisiae fermentation products as alternatives to antibiotics in finishing beef steersPubmed: 31410465
  • Lactobacillus reuteri V3401 Reduces Inflammatory Biomarkers and Modifies the Gastrointestinal Microbiome in Adults with Metabolic Syndrome: The PROSIR StudyPubmed: 31370223
  • Effects of sinomenine in LPS‐associated diseases are related to inhibition of LBP, Mac‐1, and L‐selectin levelsPubmed: 31490576
  • Needle-shaped amyloid deposition in rat mammary gland: evidence of a novel amyloid fibril proteinPubmed: 31615282
  • Ethanol Extract of Propolis Prevents High-Fat Diet-Induced Insulin Resistance and Obesity in Association with Modulation of Gut Microbiota in MicePubmed: 32156386
  • Gut microbiota from coronary artery disease patients contributes to vascular dysfunction in mice by regulating bile acid metabolism and immune activationPubmed: 33036625
  • Oxidation of fish oil exacerbates alcoholic liver disease by enhancing intestinal dysbiosis in micePubmed: 32879433
  • Antibiotic Treatment Reduces the Health Benefits of Soy ProteinPubmed: 32729948
  • Highly Branched RG-I Domain Enrichment are Indispensable for Pectin Mitigating Against High-Fat Diet-Induced ObesityPubmed: 32633953
  • Molecular characterization and expression analysis of chemokine (CXCL12) from Nile tilapia (Oreochromis niloticus)Pubmed: 32540504
  • Tandem Mass Tag (TMT)-based quantitative proteomics reveals potential targets associated with onset of Sub-clinical Mastitis in cowsPubmed: 32518370
  • Corneal epithelial injury-induced norepinephrine promotes Pseudomonas aeruginosa keratitisPubmed: 32376471
  • Naringin Attenuates High Fat Diet Induced Non-alcoholic Fatty Liver Disease and Gut Bacterial Dysbiosis in Mice
  • Umbilical cord mesenchymal stem cells (hUCMSCs) for inflammatory regulation after excision and grafting of severe burn wounds in rats33313794
  • Processing Index of Barley Grain and Dietary Undigested Neutral Detergent Fiber Concentration Affected Chewing Behavior, Ruminal pH and Total Tract Nutrient?¡­33474564
  • Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats33497213
  • Melatonin alleviates Ochratoxin A-induced liver inflammation involved intestinal microbiota homeostasis and intestinal microbiota-independent manner33582472
  • Studies on Mastitis Caused by Translocated-Bacterial Components in Ruminants
  • Monocyte transcriptomes from patients with axial spondyloarthritis reveal dysregulated monocytopoiesis and a distinct inflammatory imprint34560894
  • Composition and Functions of the Gut Microbiome in Pediatric Obesity: Relationships with Markers of Insulin Resistance34361925
  • The Prebiotic Potential of Geraniin and Geraniin-Enriched Extract against High-Fat-Diet-Induced Metabolic Syndrome in Sprague Dawley RatsPubmed:35453317
  • Elucidating the role of the gut microbiota in the physiological effects of dietary fiberPubmed:35562794
  • Local free fatty acids trigger the expression of lipopolysaccharide-binding protein in murine white adipose tissuePubmed:35433160
  • Сorrelation between the level of 25 (OH) D3 in blood, the content of antimicrobial peptides in oral fluid and dental caries intensity in young individuals

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