Multiplex Assay Kit for Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) ,etc. by CBA (Cytometric Bead Array) 
Overview
Properties
- Product No.CMX132Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryDevelopmental science
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 87-98 | 91 |
| EDTA plasma(n=5) | 82-96 | 90 |
| heparin plasma(n=5) | 84-92 | 87 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-102% | 94-103% | 98-105% | 91-98% |
| EDTA plasma(n=5) | 79-102% | 80-89% | 87-94% | 93-101% |
| heparin plasma(n=5) | 79-97% | 91-98% | 78-89% | 81-99% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) ,etc. by CBA (Cytometric Bead Array) and unlabeled Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) ,etc. by CBA (Cytometric Bead Array) in the sample. Flow Cytometer was used to determine and calculate the sample concentration.
Giveaways
Increment services
Citations
- Circulating MOTS‐c levels are decreased in obese male children and adolescents and associated with insulin resistancePubmed:29691953
- Development of a mass spectrometry‐based detection method for the mitochondrion‐derived peptide MOTS‐c in plasma samples for doping control purposesPubmed: 30394592
- Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via …Pubmed: 32880686
- Induction of hyperandrogenism and insulin resistance differentially modulates ferroptosis in uterine and placental tissues of pregnant rats
- Neural cell©\derived plasma exosome protein abnormalities implicate mitochondrial impairment in first episodes of psychosis33454965
- MOTS-c interacts synergistically with exercise intervention to regulate PGC-1¦Á expression, attenuate insulin resistance and enhance glucose metabolism in mice via?¡33722744
- Evolution of Mitochondrial-Derived Peptides, Humanin and MOTSc and Changes in Insulin Sensitivity During Early Gestation
- Abnormal levels of mitochondrial proteins in plasma neuronal extracellular vesicles in major depressive disorder34471251
- The Mitochondrial-Derived Peptide MOTS-c Relieves Hyperglycemia and Insulin Resistance in Gestational Diabetes Mellitus34798268
- SARS‐CoV‐2 and mitochondrial proteins in neural‐derived exosomes of COVID‐19Pubmed:35285072
- Evolution of Mitochondrially Derived Peptides Humanin and MOTSc, and Changes in Insulin Sensitivity during Early Gestation in Women with and without Gestational …Pubmed:35683389