Multiplex Assay Kit for Nesfatin 1 (NES1) ,etc. by CBA (Cytometric Bead Array) 
Overview
Properties
- Product No.CMA242Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- Category
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Nesfatin 1 (NES1) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Nesfatin 1 (NES1) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 86-97 | 93 |
| EDTA plasma(n=5) | 91-98 | 94 |
| heparin plasma(n=5) | 88-96 | 92 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nesfatin 1 (NES1) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nesfatin 1 (NES1) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Nesfatin 1 (NES1) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-89% | 93-101% | 88-104% | 78-95% |
| EDTA plasma(n=5) | 78-94% | 80-104% | 96-105% | 88-102% |
| heparin plasma(n=5) | 83-91% | 84-97% | 96-103% | 90-99% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:NES1) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Nesfatin 1 (NES1) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Nesfatin 1 (NES1) ,etc. by CBA (Cytometric Bead Array) and unlabeled Nesfatin 1 (NES1) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Nesfatin 1 (NES1) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Nesfatin 1 (NES1) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Nesfatin 1 (NES1) ,etc. by CBA (Cytometric Bead Array) in the sample.
Giveaways
Increment services
Citations
- Plasma Nesfatin-1 Level May Be Associated with Disease Severity in Patients with Panic DisorderPsikofarmakoloji: 2043
- High plasma nesfatin-1 level in patients with major depressive disorderPubMed: 21163317
- Cord blood nesfatin-1 and apelin-36 levels in gestational diabetes mellitusSpringerLink: h978088657555w16
- Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsive Disorder Psikofarmakoloji: 2212
- Decreased plasma nesfatin-1 levels in patients with generalized anxiety disorderPubMed: 22595767
- Effects of Aerobic Training, with or without Zizyphus Jujuba Water Extraction, on Fundus Nesfatin-1, ATP, HDL-C, and LDL-C Concentrations in Female RatsIjvst: Source
- Decreased serum nesfatin-1 levels in endometriosisPubmed: 24702902
- Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsie Disorder.Psikofarmakoloji: Source
- Role of NUCB2/nesfatin-1 as a possible biomarker.Pubmed: 23537082
- A role of nesfatin-1/NucB2 in dehydration-induced anorexiaPubmed:24829503
- Plasma nesfatin-1 levels are increased in patients with polycystic ovary syndromePubmed:24920281
- The potential role of BMI, plasma leptin, nesfatin-1 and ghrelin levels in the early detection of pancreatic necrosis and severe acute pancreatitis: a prospective cohort study.Pubmed:25448651
- The effects of short-term high-intensity interval training vs. moderate-intensity continuous training on plasma levels of nesfatin-1 and inflammatory markersPubmed:25581765
- New hormones to predict the severity of gallstone-induced acute pancreatitisPubmed:25599787
- Is There a Relationship between Obstructive Sleep Apnea Syndrome Severity and Nesfatin-1?PubMed: 26088934
- Correlation of Brain Neuropeptide (Nesfatin-1 and Orexin-A) Concentrations with Anthropometric and Biochemical Parameters in Malnourished ChildrenPubMed: 26831553
- Nesfatin-1 and Vitamin D levels may be associated with systolic and diastolic blood pressure values and hearth rate in polycystic ovary syndromePubMed: 26295295
- Relationship and Interaction between Anaerobic Sports Branches and Serum Nesfatin-1Sayilar: 294
- Nesfatin-1 levels and metabolic markers in polycystic ovary syndromePubMed: 26062107
- Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage*Pubmed:26872406
- Comparing the nesfatin-1 levels between individuals with subjective cognitive decline and mild cognitive impairmentP.1.e.012.pdf
- Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage.pubmed:26872406
- Metabolic and Cardiovascular Actions of Nesfatin-1: Implications in Health and Diseasepubmed:28137218
- Serum nesfatin-1 levels are decreased in pregnant women newly diagnosed with gestational diabetespubmed:28977160
- Serum NUCB2/nesfatin-1 levels in different stages of alcohol dependence: Is there a relationship with craving?pubmed:28529367
- Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγPubmed:29604590
- Nesfatin-1 functions as a switch for phenotype transformation and proliferation of VSMCs in hypertensive vascular remodelingPubmed:29627363
- Plasma nesfatin-1 level is associated with severity of depression in Chinese depressive patientsPubmed:29615007
- Mitra Zarrati, Mahsa Raji Lahiji, Eisa Salehi, Bahareh Yazdani, Elham Razmpoosh, Raheleh Shokouhi Shoormasti & Farzad ShidfarPubmed: 30232744
- An elevated serum level of nesfatin-1 may predict poor outcomes in patients with aneurysmal subarachnoid hemorrhages
- Relationshıp of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levelsPubmed: 32311618
- Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with ObesityPubmed: 33027789
- Assessing the blood concentration of new adipocytokines in patients with ischaemic stroke33283259
- Increased serum nesfatin-1 levels among adolescents diagnosed with major depressive disorder