Multiplex Assay Kit for Neuropeptide Y (NPY) ,etc. by CBA (Cytometric Bead Array) Cavia (Guinea pig ) Protein, Antibody, Assaykit, Service

NP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 605.00 US$ 864.00
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  • Multiplex Assay Kit for Neuropeptide Y (NPY) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Neuropeptide Y (NPY) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Neuropeptide Y (NPY) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Neuropeptide Y (NPY) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-89 85
EDTA plasma(n=5) 96-103 101
heparin plasma(n=5) 90-97 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuropeptide Y (NPY) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuropeptide Y (NPY) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neuropeptide Y (NPY) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-105% 98-105% 95-103% 95-103%
EDTA plasma(n=5) 80-102% 96-104% 95-105% 98-105%
heparin plasma(n=5) 87-101% 88-101% 83-95% 81-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:NPY) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Neuropeptide Y (NPY) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Neuropeptide Y (NPY) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Neuropeptide Y (NPY) ,etc. by CBA (Cytometric Bead Array) and unlabeled Neuropeptide Y (NPY) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Neuropeptide Y (NPY) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Neuropeptide Y (NPY) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Neuropeptide Y (NPY) ,etc. by CBA (Cytometric Bead Array) in the sample.

Giveaways

Citations

  • Is there any interaction of resistin and adiponectin levels with protein‐energy wasting among patients with chronic kidney diseaseOnlinelibrary: hdi.12072
  • Changes in growth pattern, leptin ghrelin and neuropeptide Y levels after adenotonsillectomy in prepubertal childrenDegruyter: Source
  • The role of adipocytokines on depressive symptoms of patients with chronic kidney diseasePubmed: 23879728
  • The relationship of plasma neuropeptide Y levels with coronary collateral development.Pubmed: 24077326
  • The relationship of plasma neuropeptide Y levels with coronary collateral developmentLww:Source
  • Possible role of serotonin and neuropeptide Y on the disruption of the reproductive axis activity by perfluorooctane sulfonatePubmed:25623392
  • High Plasma Levels of Neuropeptide Y Correlate With Good Clinical Outcome But are not Correlated to Cerebral Blood Flow or Vasospasm After Subarachnoid HemorrhagePubMed: 25955865
  • High Intensity Aerobic Exercise Training Improves Deficits of Cardiovascular Autonomic Function in a Rat Model of Type 1 Diabetes Mellitus with Moderate …Pubmed:26885531
  • Binge eating and biochemical markers of appetite in new users of the contraceptive depot medroxyprogesterone acetatepubmed:27604241
  • Effects of Di-isononyl Phthalate on Neuropeptide Y Expression in Differentiating Human Neuronal Cells.pubmed:27625336
  • Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor ratpubmed:27685769
  • Nur77 protects against adverse cardiac remodelling by limiting Neuropeptide Y signalling in the sympathoadrenal-cardiac axisPubmed:29850786
  • Salivary neuropeptides, stress, and periodontitisPubmed:28914594
  • Mechanisms of sustained long-term weight loss after RYGB: α-MSH is a key factorPubmed:29685637
  • Melatonin Induces Osteoblastic Differentiation of Mesenchymal Stem Cells and Promotes Fracture Healing in a Rat Model of Femoral Fracture via Neuropeptide Y …Pubmed: 30227410
  • Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome
  • Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome.
  • Sevoflurane Induces Hippocampal Neuronal Apoptosis by Altering the Level of Neuropeptide Y in Neonatal Rats.Pubmed: 32378074
  • Serum levels of neuropeptide Y in patients with chronic schizophrenia during treatment augmentation with sarcosine (results of the double©\blind randomized?¡­33245168
  • Serotonin involvement in okadaic acid-induced diarrhoea in vivo34148100

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