Multiplex Assay Kit for Neurotrophin 3 (NT3) ,etc. by CBA (Cytometric Bead Array) 
NTF3; HDNF; NGF2; Nerve Growth Factor 2; Neurotrophic Factor
Overview
Properties
- Product No.CMA106Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryCytokineNeuro science
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Neurotrophin 3 (NT3) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Neurotrophin 3 (NT3) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-89 | 85 |
| EDTA plasma(n=5) | 86-93 | 89 |
| heparin plasma(n=5) | 96-104 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurotrophin 3 (NT3) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurotrophin 3 (NT3) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neurotrophin 3 (NT3) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-101% | 94-103% | 97-105% | 80-98% |
| EDTA plasma(n=5) | 93-105% | 97-104% | 86-93% | 78-95% |
| heparin plasma(n=5) | 87-101% | 93-101% | 85-96% | 91-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Neurotrophin 3 (NT3) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Neurotrophin 3 (NT3) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Neurotrophin 3 (NT3) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Neurotrophin 3 (NT3) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.
Giveaways
Increment services
Citations
- CaracterizaTeses: Disponiveis
- Protective effects of telmisartan and tempol on lipopolysaccharide-induced cognitive impairment, neuroinflammation, and amyloidogenesis: possible role of brain-derived neurotrophic factorpubmed:28388365
- Efecto de Diferentes Intervenciones de Ejercicio Físico Agudo Sobre Niveles Plasmáticos de BDNF, NT-3 y NT-4 en Adultos con Sobrepeso:
- Histological and functional outcomes in a rat model of hemisected spinal cord with sustained VEGF/NT-3 release from tissue-engineered graftsPubmed: 31899965
- Macrophage-derived neurotrophin-3 promotes heterotopic ossification in ratsPubmed: 31896816
- Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡33389158
- Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways33589896
- Genome?wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism34092241
- Effects of a Tea Cultivar “MK5601” on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice34193676
- Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons34712429
- Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling …Pubmed:35585377