Multiplex Assay Kit for Neutrophil gelatinase-associated lipocalin (NGAL) ,etc. by CBA (Cytometric Bead Array) Homo sapiens (Human) Protein, Antibody, Assaykit, Service

Lipocalin-2; LCN2; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN2

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 402.00 US$ 574.00
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  • Multiplex Assay Kit for Neutrophil gelatinase-associated lipocalin (NGAL) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Neutrophil gelatinase-associated lipocalin (NGAL) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Neutrophil gelatinase-associated lipocalin (NGAL) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Neutrophil gelatinase-associated lipocalin (NGAL) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 101
EDTA plasma(n=5) 85-104 94
heparin plasma(n=5) 97-104 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neutrophil gelatinase-associated lipocalin (NGAL) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neutrophil gelatinase-associated lipocalin (NGAL) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neutrophil gelatinase-associated lipocalin (NGAL) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-95% 78-93% 80-94% 95-104%
EDTA plasma(n=5) 78-103% 80-99% 96-105% 96-103%
heparin plasma(n=5) 95-102% 89-98% 84-103% 87-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Neutrophil gelatinase-associated lipocalin (NGAL) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Neutrophil gelatinase-associated lipocalin (NGAL) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Neutrophil gelatinase-associated lipocalin (NGAL) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Neutrophil gelatinase-associated lipocalin (NGAL) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Neutrophil gelatinase-associated lipocalin (NGAL) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • Correlation of serum neutrophil gelatinase-associated lipocalin with acute kidney injury in hypertensive disorders of pregnancyPubMed: PMC3794962
  • Cholesterol Contributes to Diabetic Nephropathy through SCAP-SREBP-2 PathwayPubmed: 24369464
  • Lipocalin-2: Response To Resistance Training in Obese Young MenIjssjournal: Source
  • Effects of Resistance versus Endurance Training on Plasma Lipocalin-2 in Young MenTums: Source
  • Differences in plasma and sputum biomarkers between COPD and COPD-asthma overlapPubmed: 23794464
  • Suppressor of cytokine signaling (SOCS) 2 attenuates renal lesions in rats with diabetic nephropathyPubmed:24802166
  • Nephrotoxic effects of varenicline as the most effective drug used for smoking cessation: a preliminary experimental studyPubmed:25812822
  • 血清及胆汁中NGAL 对于区分良恶性胆道狭窄的临床意义Wjgnet:Source
  • Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty ratsSagepub:Source
  • Hs-CRP and Adipokin (Lcn2): Response to Exercise Training in Obese MenBiomedpharmajournal:Source
  • A Study On Thyroid Hormone And Its Effects On Exercise Ifcss:Source
  • 中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18 在诊断心肺分流术后急性肾损伤中的意义Jcp-Sh:Source
  • Correlation of serum neutrophil gelatinase associated lipocalin with disease severity in hypertensive disorders of pregnancyPubmed:Pmc4260273
  • Increased serum levels of lipocalin-1 and-2 in patients with stable chronic obstructive pulmonary diseasePubmed:Pmc4043430
  • Impact of 6% hydroxyethyl starch 130/0.42 and 4% gelatin on renal function in a pediatric animal modelPubmed:24916275
  • Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous CrisisPubmed:25448261
  • Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitisPubMed: 26191125
  • Increased cardiac index attenuates septic acute kidney injury: a prospective observational studyPubMed: 25745357
  • 中性粒细胞明胶酶相关脂质运载蛋白对腹膜透析相关性腹膜炎疗效监测的意义Journal: Fulltext
  • Ameliorative Effect of Chrysin on Adenine-Induced Chronic Kidney Disease in RatsPubMed: 25909514
  • Impact of high doses of 6% hydroxyethyl starch 130/0.42 and 4% gelatin on renal function in a pediatric animal modelPubmed:26714833
  • From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases.Pubmed:26991748
  • Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrolpublication:299492265_Diabetes-induced_renal_failu
  • The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in ratspubmed:27430552
  • Hyperoxidized albumin modulates neutrophils to induce oxidative stress and inflammation in severe alcoholic hepatitis.pubmed:27775820
  • Advanced glycation end products (AGEs) increase renal lipid accumulation: a pathogenic factor of diabetic nephropathy (DN)pubmed:28659153
  • From varices to venous ulceration: the story of chronic venous disease described by metalloproteinasespubmed:26991748
  • Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats.pubmed:29027259
  • Hemojuvelin Predicts Acute Kidney Injury and Poor Outcomes Following Cardiac SurgeryPubmed:29386545
  • Pyrrolidine dithiocarbamate inhibits mouse acute kidney injury induced by diclofenac by targeting oxidative damage, cytokines and NF-κB activityPubmed:30036488
  • Effect of Pinocembrin Isolated from Mexican Brown Propolis on Diabetic NephropathyPubmed:29642511
  • Improved approach for normothermic machine perfusion of cold stored kidney graftsPubmed:30018731
  • Chronic obstructive pulmonary disease (COPD)--from biomarkers to clinical phenotypesISBN:978-951-51-4039-5
  • Potential Novel Biomarkers of Obstructive Nephropathy in Children with HydronephrosisDoi: 10.1155/2018/1015726
  • The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic NephropathyPubmed: 30504713
  • Dual actions on gout flare and acute kidney injury along with enhanced renal transporter activities by Yokuininto, a Kampo medicinePubmed: 30871515
  • Piceatannol protects against cisplatin nephrotoxicity via activation of Nrf2/HO-1 pathway and hindering NF-κB inflammatory cascadePubmed: 31197431
  • A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activationPubmed: 31447572
  • Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixturesPubmed: 31639605
  • Alkaline phosphatase treatment of Acute Kidney injury in an infant piglet Model of cardiopulmonary Bypass with Deep Hypothermic circulatory ArrestPubmed: 31578351
  • The evaluation of acute kidney injury due to ischemia by urinary neutrophil gelatinase-induced lipocalin (uNGAL) measurement in patients who underwent partial …Pubmed: 32980930
  • Upregulated miR-101 inhibits acute kidney injury–chronic kidney disease transition by regulating epithelial–mesenchymal transitionPubmed: 32633566
  • CОСТОЯНИЕ ТИРЕОИДНОЙ ФУНКЦИИ У ПАЦИЕНТОВ С САХАРНЫМ ДИАБЕТОМ 1-ГО ТИПА И ХРОНИЧЕСКОЙ БОЛЕЗНЬЮ ПОЧЕК …
  • Thyroid function in patients with type 1 diabetes mellitus and chronic kidney disease receiving renal replacement therapy
  • Early Kidney Injury in Immunoglobulin A Vasculitis: Role of Renal Biomarkers33423326
  • Nrf2 deficiency aggravates the kidney injury induced by subacute cadmium exposure in mice33398418
  • Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury33604384
  • Elevated plasma level of neutrophil gelatinase-associated lipocalin (NGAL) in patients with breast cancer34104101
  • Preservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental …34169407
  • Preservation of Mitochondrial Coupling and Renal Function by Controlled Oxygenated Rewarming of Porcine Kidney Grafts34944524
  • SERS based Y-shaped aptasensor for early diagnosis of acute kidney injuryPubmed:35733690

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