Multiplex Assay Kit for Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) ,etc. by CBA (Cytometric Bead Array) 
MOX1; GP91-2; NOH1; NADPH Oxidase 1; Mitogenic Oxidase(Pyridine Nucleotide-Dependent Superoxide-Generating; NADH/NADPH mitogenic oxidase subunit P65-MOX
Overview
Properties
- Product No.CMA554Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & KinaseNeuro scienceDevelopmental science
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 90-97 | 94 |
| EDTA plasma(n=5) | 84-104 | 92 |
| heparin plasma(n=5) | 83-91 | 87 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-105% | 96-104% | 83-90% | 89-98% |
| EDTA plasma(n=5) | 84-105% | 92-104% | 87-98% | 78-95% |
| heparin plasma(n=5) | 98-105% | 80-99% | 87-96% | 82-89% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.
Giveaways
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Citations
- Pre-treatment with cardamonin protects against cisplatin-induced nephrotoxicity in rats: Impact on NOX-1, inflammation and apoptosisPubmed: 24211271
- Apocynin protects against ethanol-induced gastric ulcer in rats by attenuating the upregulation of NADPH oxidases 1 and 4PubMed: 26522475
- RESISTANCE OR AEROBIC TRAINING DECREASES BLOOD PRESSURE AND IMPROVES CARDIOVASCULARAUTONOMIC CONTROL AND OXIDATIVE STRESS IN HYPERTENSIVE MENOPAUSAL RATS.Pubmed:27339182
- Indole-3- carbinol enhances sorafenib cytotoxicity in hepatocellular carcinoma cells: A mechanistic studypubmed:27612096
- Indole-3-carbinol protects against cisplatin-induced acute nephrotoxicity: role of calcitonin gene-related peptide and insulin-like growth factor-1.pubmed:27417335
- Camel Milk Ameliorates 5-Fluorouracil-Induced Renal Injury in Rats: Targeting MAPKs, NF-κB and PI3K/Akt/eNOS PathwaysPubmed:29694984
- Camel milk attenuates methotrexate-induced kidney injury via activation of PI3K/Akt/eNOS signaling and intervention with oxidative aberrationsPubmed:29667662
- Anti-inflammatory and cytoprotective effect of plant sterol and galactooligosaccharides enriched beverages in Caco-2 cellsPubmed: 31290324
- NOX5-induced uncoupling of endothelial NO synthase is a causal mechanism and theragnostic target of an age-related hypertension endotypePubmed: 33170835
- Targeting oxidative stress, apoptosis, and autophagy by galangin mitigates cadmium-induced renal damage: Role of SIRT1/Nrf2 and AMPK/mTOR pathwaysPubmed:34999115
- Targeting inflammation, autophagy, and apoptosis by troxerutin attenuates methotrexate-induced renal injury in ratsPubmed:34953450