Multiplex Assay Kit for Osteoprotegerin (OPG) ,etc. by CBA (Cytometric Bead Array) Rattus norvegicus (Rat) Protein, Antibody, Assaykit, Service

TNFRSF11B; TNFRSF11-B; OCIF; OPG; TR1; Tumor Necrosis Factor Receptor Superfamily Member 11b; Osteoclastogenesis Inhibitory Factor

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 575.00 US$ 821.00
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  • Multiplex Assay Kit for Osteoprotegerin (OPG) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Osteoprotegerin (OPG) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Osteoprotegerin (OPG) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Osteoprotegerin (OPG) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-97 94
EDTA plasma(n=5) 82-104 96
heparin plasma(n=5) 83-101 87

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteoprotegerin (OPG) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteoprotegerin (OPG) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Osteoprotegerin (OPG) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-99% 99-105% 80-96% 92-101%
EDTA plasma(n=5) 79-102% 97-105% 80-101% 93-104%
heparin plasma(n=5) 98-105% 86-93% 81-91% 95-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Osteoprotegerin (OPG) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Osteoprotegerin (OPG) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Osteoprotegerin (OPG) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Osteoprotegerin (OPG) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Osteoprotegerin (OPG) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • Protective Effect of Amlodipine on Rat Bone Tissue after OrchidectomyKarger: 335491
  • Protective effect of atorvastatin on bone tissue in orchidectomised male albino Wistar ratsScienceDirect: S0014299912000659
  • Adipokines in Psoriatic Arthritis Patients: The Correlations with Osteoclast Precursors and Bone ErosionsPubMed: PMC3483160
  • The effects of chronic zoledronate usage on the jaw and long bones evaluated using RANKL and osteoprotegerin levels in an animal modelPubmed: 23522850
  • OPG and RANKL levels around miniscrew implants during orthodontic tooth movementPubmed: 23910201
  • Estrogen deficiency does not decrease the in vitro osteogenic potential of rat adipose-derived mesenchymal stem cellsPubmed: 24687841
  • Effects and mechanisms of 8-prenylnaringenin on osteoblast MC3T3-E1 and osteoclast-like cells RAW264.7Onlinelibrary: fsn3.109
  • Effects and mechanisms of 8‐prenylnaringenin on osteoblast MC3T3‐E1 and osteoclast‐like cells RAW264. 7Pubmed:Pmc4221832
  • The effect of topiramate and lamotrigine on rat bone mass, structure and metabolism.Pubmed:24629477
  • Effect of Nicotine on RANKL and OPG and Bone Mineral DensityPubmed:24830656
  • Effects of icariin on the regulation of the OPG?RANKL?RANK system are mediated through the MAPK pathways in IL?1β?stimulated human SW1353 chondrosarcoma …Pubmed:25270538
  • Carvedilol promotes neurological function, reduces bone loss and attenuates cell damage after acute spinal cord injury in ratsPubmed:25424914
  • Strontium and zoledronate hydroxyapatites graded composite coatings for bone prosthesesPubmed:25706198
  • A novel model of bisphosphonate-related osteonecrosis of the jaw in ratsPubMed: 26191212
  • Relation of Osteoprotegerin, Visfatin and Ghrelin to Metabolic Syndrome in Type 2 Diabetic PatientsPubMed: 26309431
  • Effect of Mirtazapine on Rat Bone Tissue after OrchidectomyPubMed: 25871861
  • Antiosteoporotic effect of icariin in ovariectomized rats is mediated via the Wnt/β-catenin pathwayPubmed:27347050
  • The effects of strength training and raloxifene on bone health in aging ovariectomized ratsPubmed:26812611
  • Effetti del laser a bassa dose su cellule osteoblasticheINCERTIPARENTI_SERENA_TESI.pdf
  • RAW 264.7 co-cultured with ultra-high molecular weight polyethylene particles spontaneously differentiate into osteoclasts: an in vitro model of periprosthetic osteolysis.pubmed:27667508
  • Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats.pubmed:27908549
  • Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats.pubmed:28158228
  • Skeletal Site-specific Effects of Zoledronate on in vivo Bone Remodeling and in vitro BMSCs Osteogenic Activity.pubmed:28139685
  • Modification and Characterization of Polyethylene Glycolated Decellularized Porcine Aortic Heart Valve with Osteoprotegerinart00007
  • Allogeneic yet major histocompatibility complex‑matched bone marrow transplantation in mice results in an impairment of osteoblasts and a significantly reduced trabecular bonearticle:10.1007
  • Assessment of bone metabolism and biomechanical properties of the femur, following treatment with anastrozole and letrozole in an experimental model of menopauseol.2017.6596
  • RAW 264.7 co-cultured with ultra-high molecular weight polyethylene particles spontaneously differentiate into osteoclasts: an in vitro model of periprosthetic osteolysis10.1002/jbm.a.35912
  • Mesenchymal stem cells inhibit RANK-RANKL interactions between osteoclasts and Th17 cells via osteoprotegerin activity.pubmed:29137353
  • A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/β-catenin Signaling Pathways in Ovariectomized RatsISSN 2249 – 622X
  • Enhanced Osseointegration of Porous Titanium Modified with Zeolitic Imidazolate Framework-8.pubmed:28696091
  • Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in ratsPubmed:29574908
  • Effect of long-term administration of mangiferin from on bone metabolism in ovariectomized rats10.1016:j.jff.2018.04.048
  • Strontium‐Substituted Hydroxyapatite‐Gelatin Biomimetic Scaffolds Modulate Bone Cell ResponsePubmed:29877029
  • Effects of water extract from epimedium on neuropeptide signaling in an ovariectomized osteoporosis rat modelPubmed:29705515
  • Bu‑Shen‑Ning‑Xin decoction suppresses osteoclastogenesis by modulating RANKL/OPG imbalance in the CD4+ T lymphocytes of ovariectomized micePubmed:29717766
  • Allogeneic yet major histocompatibility complex-matched bone marrow transplantation in mice results in an impairment of osteoblasts and a significantly reduced …Pubmed:28766138
  • Therapeutic effect of icariin combined with stem cells on postmenopausal osteoporosis in ratsPubmed:28681147
  • Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive RatsPubmed:29898457
  • Liraglutide exerts a bone‑protective effect in ovariectomized rats with streptozotocin‑induced diabetes by inhibiting osteoclastogenesisPubmed:29805533
  • The sole and combined effect of simvastatin and platelet rich fibrin as a filling material in induced bone defect in tibia of albino ratsPubmed: 30208342
  • NUMB maintains bone mass by promoting degradation of PTEN and GLI1 via ubiquitination in osteoblastsPubmed: 30455992
  • Development and characterization of supramolecular calcitonin assembly and assessment of its interactions with the bone remodelling processPubmed: 30797058
  • Biofunctionalization of decellularized porcine aortic valve with OPG-loaded PCL nanoparticles for anti-calcificationDoi: 10.1039/C9RA00408D
  • Promoter Methylation Down-regulates Osteoprotegerin Expression in Ovarian CarcinomaPubmed: 31092428
  • IL-38 restrains inflammatory response of collagen-induced arthritis in rats via SIRT1/HIF-1α signaling pathwayPubmed: 32347300
  • Long-Term Administration of Abacavir and Etravirine Impairs Semen Quality and Alters Redox System and Bone Metabolism in Growing Male Wistar Rats34373766
  • Jiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axisPubmed:35453007
  • Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot StudyPubmed:35743980
  • Exercised accelerated the production of muscle-derived kynurenic acid in skeletal muscle and alleviated the postmenopausal osteoporosis through the Gpr35 …

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