Multiplex Assay Kit for Parathyroid Hormone Related Protein (PTHrP) ,etc. by CBA (Cytometric Bead Array) Homo sapiens (Human) Protein, Antibody, Assaykit, Service

PTHLH; HHM; PTHR; PLP; Osteostatin; Parathyroid Hormone Like Hormone

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  • Multiplex Assay Kit for Parathyroid Hormone Related Protein (PTHrP) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Parathyroid Hormone Related Protein (PTHrP) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Parathyroid Hormone Related Protein (PTHrP) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Parathyroid Hormone Related Protein (PTHrP) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-101 97
EDTA plasma(n=5) 91-103 95
heparin plasma(n=5) 96-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Parathyroid Hormone Related Protein (PTHrP) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Parathyroid Hormone Related Protein (PTHrP) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Parathyroid Hormone Related Protein (PTHrP) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-99% 97-105% 93-101% 92-101%
EDTA plasma(n=5) 83-91% 80-104% 98-105% 90-97%
heparin plasma(n=5) 92-101% 91-99% 90-103% 85-94%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:PTHrP) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Parathyroid Hormone Related Protein (PTHrP) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Parathyroid Hormone Related Protein (PTHrP) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Parathyroid Hormone Related Protein (PTHrP) ,etc. by CBA (Cytometric Bead Array) and unlabeled Parathyroid Hormone Related Protein (PTHrP) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Parathyroid Hormone Related Protein (PTHrP) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Parathyroid Hormone Related Protein (PTHrP) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Parathyroid Hormone Related Protein (PTHrP) ,etc. by CBA (Cytometric Bead Array) in the sample.

Giveaways

Citations

  • Characterization of a new renal cell carcinoma bone metastasis mouse modelPubMed: 20443133
  • Temporal activation of β-catenin signaling in the chondrogenic process of mesenchymal stem cells affects the phenotype of the cartilage generated.PubMed: 22133004
  • PTHrP Expression in Human MDA-MB-231 Breast Cancer Cells Is Critical for Tumor Growth and Survival and Osteoblast InhibitionPubMed: PMC3753447
  • Tetrahydrofurofuran-type lignans inhibit breast cancer-mediated bone destruction by blocking the vicious cycle between cancer cells, osteoblasts and osteoclastsPubmed: 23673814
  • Betulinic acid, a bioactive pentacyclic triterpenoid, inhibits skeletal-related events induced by breast cancer bone metastases and treatmentPubmed:24463094
  • Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control studyPubmed:24943060
  • 15-Deoxy-Δ12, 14-Prostaglandin J2 Inhibits Osteolytic Breast Cancer Bone Metastasis and Estrogen Deficiency-Induced Bone LossPubMed: 25859665
  • A role for the calcitonin receptor to limit bone loss during lactation in female mice by inhibiting osteocytic osteolysisPubMed: 26135836
  • Osteopontin expression in co-cultures of human squamous cell carcinoma-derived cells and osteoblastic cells and its effects on the neoplastic cell phenotype and osteoclastic activation.Pubmed:27312487
  • Hedgehog signaling in bone regulates whole-body energy metabolism through a bone–adipose endocrine relay mediated by PTHrP and adiponectinpubmed:27740628
  • Lactation induces increases in the RANK/RANKL/OPG system in maxillary bonePubmed:29382610
  • A Diet Rich in Fish Oil and Leucine Ameliorates Hypercalcemia in Tumour-Induced Cachectic MicePubmed: 31600911
  • Identification of a potential gene target for osteoarthritis based on bioinformatics analysesPubmed: 32571421
  • Parathyroid hormone related protein (PTHrP)-mediated hypercalcemia in malignancy associated with anti-PD-1 immune checkpoint inhibitor treatment and related …
  • Паратиреоидный гормон-родственный протеин как предиктор развития мочекаменной болезни
  • Extracellular vesicles-released parathyroid hormone-related protein from Lewis lung carcinoma induces lipolysis and adipose tissue browning in cancer ¡­33510128
  • Effects of whole-body vibration training in a cachectic C26 mouse model34732809
  • The acute adaptation of renal phosphate transporters in the murine kidney to oral phosphate intake requires multiple signalsPubmed:35334154

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